Tombusviruses upregulate phospholipid biosynthesis via interaction between p33 replication protein and yeast lipid sensor proteins during virus replication in yeast

Daniel Barajas, Kai Xu, Monika Sharma, Cheng Yu Wu, Peter D. Nagy

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Positive-stranded RNA viruses induce new membranous structures and promote membrane proliferation in infected cells to facilitate viral replication. In this paper, the authors show that a plant-infecting tombusvirus upregulates transcription of phospholipid biosynthesis genes, such as INO1, OPI3 and CHO1, and increases phospholipid levels in yeast model host. This is accomplished by the viral p33 replication protein, which interacts with Opi1p FFAT domain protein and Scs2p VAP protein. Opi1p and Scs2p are phospholipid sensor proteins and they repress the expression of phospholipid genes. Accordingly, deletion of OPI1 transcription repressor in yeast has a stimulatory effect on TBSV RNA accumulation and enhanced tombusvirus replicase activity in an in vitro assay. Altogether, the presented data convincingly demonstrate that de novo lipid biosynthesis is required for optimal TBSV replication. Overall, this work reveals that a (+)RNA virus reprograms the phospholipid biosynthesis pathway in a unique way to facilitate its replication in yeast cells.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalVirology
Volume471-473
DOIs
StatePublished - Dec 1 2014

Bibliographical note

Publisher Copyright:
© 2014 Elsevier Inc.

Keywords

  • FFAT domain
  • Membrane proliferation
  • Phosphatidic acid
  • Phospholipids
  • Replicase
  • Replication in vitro
  • Tomato bushy stunt virus
  • Transcription repressor
  • VAP domain
  • Yeast host

ASJC Scopus subject areas

  • Virology

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