Here we report a novel microelectrode array recording approach to measure tonic (resting) and phasic release of dopamine (DA) in DA-rich areas such as the rat striatum and nucleus accumbens. The resulting method is tested in intact central nervous system (CNS) and in animals with extensive loss of the DA pathway using the neurotoxin, 6-hydroxyDA (6-OHDA). The self-referencing amperometric recording method employs Nafion-coated with and without m-phenylenediamine recording sites that through real-time subtraction allow for simultaneous measures of tonic DA levels and transient changes due to depolarization and amphetamine-induced release. The recording method achieves low-level measures of both tonic and phasic DA with decreased recording drift allowing for enhanced sensitivity normally not achieved with electrochemical sensors in vivo.
|Journal||Applied Sciences (Switzerland)|
|State||Published - Sep 2020|
Bibliographical noteFunding Information:
Funding: This research was funded by NIH CEBRA II DA017186. M.L. was supported by Wenner-Gren Foundation, Stockholm, Sweden.
This research was funded by NIH CEBRA II DA017186. M.L. was supported by Wenner-Gren Foundation, Stockholm, Sweden.
© 2020 by the authors.
- Microelectrode array
ASJC Scopus subject areas
- Materials Science (all)
- Engineering (all)
- Process Chemistry and Technology
- Computer Science Applications
- Fluid Flow and Transfer Processes