Tools and methods for studying the Drosophila JAK/STAT pathway

Qian Chen; Michelle Giedt; Lingfeng Tang; Douglas A. Harrison

doi:10.1016/j.ymeth.2014.03.023

Tools and methods for studying the Drosophila JAK/STAT pathway

Qian Chen, Michelle Giedt, Lingfeng Tang, Douglas A. Harrison

Biology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The Janus Kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway is one of a limited number of signaling cascades that is extensively utilized for many developmental and homeostatic functions. The JAK/STAT pathway is evolutionarily conserved from insects to mammals, with homologous transduction machinery in each. Yet the mammalian pathway is composed of multiple members for each family of proteins, while flies have only a single homologue of most pathway components. This simplicity and the abundance of genetic, biochemical, and developmental tools make Drosophila an attractive model to investigate this important signaling pathway. This review provides a basic description of the Drosophila JAK/STAT cascade and summarizes currently available reagents and tools to study and manipulate the pathway.

Original language	English
Pages (from-to)	160-172
Number of pages	13
Journal	Methods
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2014.03.023
State	Published - Jun 15 2014

Bibliographical note

Funding Information:
The authors thank Dr. S.M.W. Harrison for comments and suggestions on this manuscript. Research in the lab has been supported by the National Science Foundation ( IOB-0920432 ).

Keywords

Cell signaling
Cytokine
Drosophila
Genetics
Janus kinase (JAK)
STAT

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology (all)

Access to Document

10.1016/j.ymeth.2014.03.023

Cite this

@article{4e22b47497014eb1b603615eae8fbd39,

title = "Tools and methods for studying the Drosophila JAK/STAT pathway",

abstract = "The Janus Kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway is one of a limited number of signaling cascades that is extensively utilized for many developmental and homeostatic functions. The JAK/STAT pathway is evolutionarily conserved from insects to mammals, with homologous transduction machinery in each. Yet the mammalian pathway is composed of multiple members for each family of proteins, while flies have only a single homologue of most pathway components. This simplicity and the abundance of genetic, biochemical, and developmental tools make Drosophila an attractive model to investigate this important signaling pathway. This review provides a basic description of the Drosophila JAK/STAT cascade and summarizes currently available reagents and tools to study and manipulate the pathway.",

keywords = "Cell signaling, Cytokine, Drosophila, Genetics, Janus kinase (JAK), STAT",

author = "Qian Chen and Michelle Giedt and Lingfeng Tang and Harrison, {Douglas A.}",

note = "Funding Information: The authors thank Dr. S.M.W. Harrison for comments and suggestions on this manuscript. Research in the lab has been supported by the National Science Foundation ( IOB-0920432 ).",

year = "2014",

month = jun,

day = "15",

doi = "10.1016/j.ymeth.2014.03.023",

language = "English",

volume = "68",

pages = "160--172",

number = "1",

}

TY - JOUR

T1 - Tools and methods for studying the Drosophila JAK/STAT pathway

AU - Chen, Qian

AU - Giedt, Michelle

AU - Tang, Lingfeng

AU - Harrison, Douglas A.

N1 - Funding Information: The authors thank Dr. S.M.W. Harrison for comments and suggestions on this manuscript. Research in the lab has been supported by the National Science Foundation ( IOB-0920432 ).

PY - 2014/6/15

Y1 - 2014/6/15

N2 - The Janus Kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway is one of a limited number of signaling cascades that is extensively utilized for many developmental and homeostatic functions. The JAK/STAT pathway is evolutionarily conserved from insects to mammals, with homologous transduction machinery in each. Yet the mammalian pathway is composed of multiple members for each family of proteins, while flies have only a single homologue of most pathway components. This simplicity and the abundance of genetic, biochemical, and developmental tools make Drosophila an attractive model to investigate this important signaling pathway. This review provides a basic description of the Drosophila JAK/STAT cascade and summarizes currently available reagents and tools to study and manipulate the pathway.

AB - The Janus Kinase/Signal Transducers and Activators of Transcription (JAK/STAT) signaling pathway is one of a limited number of signaling cascades that is extensively utilized for many developmental and homeostatic functions. The JAK/STAT pathway is evolutionarily conserved from insects to mammals, with homologous transduction machinery in each. Yet the mammalian pathway is composed of multiple members for each family of proteins, while flies have only a single homologue of most pathway components. This simplicity and the abundance of genetic, biochemical, and developmental tools make Drosophila an attractive model to investigate this important signaling pathway. This review provides a basic description of the Drosophila JAK/STAT cascade and summarizes currently available reagents and tools to study and manipulate the pathway.

KW - Cell signaling

KW - Cytokine

KW - Drosophila

KW - Genetics

KW - Janus kinase (JAK)

KW - STAT

UR - http://www.scopus.com/inward/record.url?scp=84901838047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901838047&partnerID=8YFLogxK

U2 - 10.1016/j.ymeth.2014.03.023

DO - 10.1016/j.ymeth.2014.03.023

M3 - Article

C2 - 24685392

AN - SCOPUS:84901838047

VL - 68

SP - 160

EP - 172

IS - 1

ER -

Tools and methods for studying the Drosophila JAK/STAT pathway

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this