Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus

Qiji Deng, Yuejin Weng, Wuxun Lu, Andrew Demers, Minxun Song, Dan Wang, Qingzhong Yu, Feng Li

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The small hydrophobic protein (SH) is a type II integral membrane protein that is packaged into virions and is only present in certain paramyxoviruses including metapneumovirus. In addition to a highly divergent primary sequence, SH proteins vary significantly in size amongst the different viruses. Human respiratory syncytial virus (HRSV) encodes the smallest SH protein consisting of only 64 amino acids, while metapneumoviruses have the longest SH protein ranging from 174 to 179 amino acids in length. Little is currently known about the cellular localization and topology of the metapneumovirus SH protein. Here we characterize for the first time metapneumovirus SH protein with respect to topology, subcellular localization, and transport using avian metapneumovirus subgroup C (AMPV-C) as a model system. We show that AMPV-C SH is an integral membrane protein with N inC out orientation located in both the plasma membrane as well as within intracellular compartments, which is similar to what has been described previously for SH proteins of other paramyxoviruses. Furthermore, we demonstrate that AMPV-C SH protein localizes in the endoplasmic reticulum (ER), Golgi, and cell surface, and is transported through ER-Golgi secretory pathway.

Original languageEnglish
Pages (from-to)102-107
Number of pages6
JournalVirus Research
Issue number1-2
StatePublished - Sep 2011

Bibliographical note

Funding Information:
We thank Elizabeth Kolb for editing the manuscript and Xiuqin Xia for outstanding technical assistance. This work was supported by the SD AES Fund (3AH-203) to FL, and USDA, ARS CRIS project 6612-32000-056-00D (to QY).


  • Avian metapneumovirus
  • Subcellular localization
  • Topology
  • Transport

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases


Dive into the research topics of 'Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus'. Together they form a unique fingerprint.

Cite this