Toward biochemical conversion of lignocellulose on-farm: pretreatment and hydrolysis of corn stover in situ

A. A. Modenbach; S. E. Nokes; M. D. Montross; B. L. Knutson

doi:10.13031/trans.12069

Toward biochemical conversion of lignocellulose on-farm: pretreatment and hydrolysis of corn stover in situ

A. A. Modenbach, S. E. Nokes, M. D. Montross, B. L. Knutson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an in-situ on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment and hydrolysis. Increasing the enzyme dosage improved cellulose conversion from 9.6% to 36.8% when high-solids loadings were used in both unit operations; however, increasing NaOH loading and pretreatment time did not increase the conversion efficiency. The enzyme-to-substrate ratio had a larger impact on cellulose conversion than the NaOH pretreatment conditions studied, resulting in recommendations for an on-farm bioconversion system.

Original language	English
Pages (from-to)	1025-1033
Number of pages	9
Journal	Transactions of the ASABE
Volume	60
Issue number	4
DOIs	https://doi.org/10.13031/trans.12069
State	Published - 2017

Bibliographical note

Publisher Copyright:
© 2017 American Society of Agricultural and Biological Engineers.

Funding

The authors gratefully acknowledge the financial support of the USDA-NIFA Biomass Research and Development Initiative (Grants No. 11000000836 and No. 2011-10006-30363). The investigation reported in this study is a part of a project of the Kentucky Agricultural Experiment Station (No. 16-05-087) and is published with the approval of the director.

Funders	Funder number
USDA NIFA Biomass Research and Development Initiative Program	2011-10006-30363, 11000000836

Keywords

Corn stover
Enzymatic hydrolysis
Enzyme loading
High solids
Low solids
Sodium hydroxide

ASJC Scopus subject areas

Forestry
Food Science
Biomedical Engineering
Agronomy and Crop Science
Soil Science

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10.13031/trans.12069

Cite this

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abstract = "High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an in-situ on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment and hydrolysis. Increasing the enzyme dosage improved cellulose conversion from 9.6% to 36.8% when high-solids loadings were used in both unit operations; however, increasing NaOH loading and pretreatment time did not increase the conversion efficiency. The enzyme-to-substrate ratio had a larger impact on cellulose conversion than the NaOH pretreatment conditions studied, resulting in recommendations for an on-farm bioconversion system.",

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AU - Nokes, S. E.

AU - Montross, M. D.

AU - Knutson, B. L.

PY - 2017

Y1 - 2017

N2 - High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an in-situ on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment and hydrolysis. Increasing the enzyme dosage improved cellulose conversion from 9.6% to 36.8% when high-solids loadings were used in both unit operations; however, increasing NaOH loading and pretreatment time did not increase the conversion efficiency. The enzyme-to-substrate ratio had a larger impact on cellulose conversion than the NaOH pretreatment conditions studied, resulting in recommendations for an on-farm bioconversion system.

AB - High-solids lignocellulosic pretreatment using NaOH followed by high-solids enzymatic hydrolysis was evaluated for an on-farm biochemical conversion process. Increasing the solids loadings for these processes has the potential for increasing glucose concentrations and downstream ethanol production; however, sequential processing at high-solids loading similar to an in-situ on-farm cellulose conversion system has not been studied. This research quantified the effects of high-solids pretreatment with NaOH and subsequent high-solids enzymatic hydrolysis on cellulose conversion. As expected, conversion efficiency was reduced; however, the highest glucose concentration (40.2 g L-1), and therefore the highest potential ethanol concentration, resulted from the high-solids combined pretreatment and hydrolysis. Increasing the enzyme dosage improved cellulose conversion from 9.6% to 36.8% when high-solids loadings were used in both unit operations; however, increasing NaOH loading and pretreatment time did not increase the conversion efficiency. The enzyme-to-substrate ratio had a larger impact on cellulose conversion than the NaOH pretreatment conditions studied, resulting in recommendations for an on-farm bioconversion system.

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KW - Low solids

KW - Sodium hydroxide

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Toward biochemical conversion of lignocellulose on-farm: pretreatment and hydrolysis of corn stover in situ

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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