Toxicity of ecdysone agonists correlates with the induction of CHR3 mRNA in the spruce budworm

M. Sundaram; S. R. Palli; I. Ishaaya; P. J. Krell; A. Retnakaran

doi:10.1006/pest.1998.2383

Toxicity of ecdysone agonists correlates with the induction of CHR3 mRNA in the spruce budworm

M. Sundaram
, S. R. Palli
, I. Ishaaya
, P. J. Krell
, A. Retnakaran

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Four ecdysone agonists, RH-5849, RH-5992, RH-0345, and RH-2485, were tested for toxicity against fifth-instar larvae of Choristoneura fumiferana. RH-2485 was the most toxic (LD⁵⁰ = 1.05 ng/larva) followed by RH-5992 (LD⁵⁰ = 8.8 ng/larva), RH-0345 (LD⁵⁰ = 41.0 ng/larva), and RH-5849 (LD⁵⁰ = 236.9 ng/larva). The relative potencies of these compounds to induce the transcription factor Choristoneura hormone receptor 3 (CHR3) was studied. Detectable levels of CHR3 mRNA were induced by RH-2485 at a concentration as low as 1.5 ng/larva, but similar inductions required concentrations as high as 7.5 ng of RH-5992/larva, 37.5 of RH-0345/larva, and 187.5 of RH-5849/larva. The order of potency of these four ecdysone agonists for inducing CHR3 mRNA is the same as their larval toxicity.

Original language	English
Pages (from-to)	201-208
Number of pages	8
Journal	Pesticide Biochemistry and Physiology
Volume	62
Issue number	3
DOIs	https://doi.org/10.1006/pest.1998.2383
State	Published - Dec 1998

Bibliographical note

Funding Information:
We thank Rohm and Haas company scientists for supplying compounds, Dr. Sardar S. Sohi for critical comments on the manuscript, Ms. K. Jamieson for editorial help. This research was supported by a Canadian Forest Service/Natural Sciences and Engineering Research Council Research Partnership Program Award CRD No. 0192158, with Rohm and Haas Company.

Funding

We thank Rohm and Haas company scientists for supplying compounds, Dr. Sardar S. Sohi for critical comments on the manuscript, Ms. K. Jamieson for editorial help. This research was supported by a Canadian Forest Service/Natural Sciences and Engineering Research Council Research Partnership Program Award CRD No. 0192158, with Rohm and Haas Company.

Funders	Funder number
Canadian Forest Service/Natural Sciences and Engineering Research Council	0192158

Keywords

Hormone receptor 3
Pest control
RH-0345
RH-2485
RH-5849
RH-5992
Spruce budworm
Transcription factor

ASJC Scopus subject areas

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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10.1006/pest.1998.2383

Cite this

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title = "Toxicity of ecdysone agonists correlates with the induction of CHR3 mRNA in the spruce budworm",

abstract = "Four ecdysone agonists, RH-5849, RH-5992, RH-0345, and RH-2485, were tested for toxicity against fifth-instar larvae of Choristoneura fumiferana. RH-2485 was the most toxic (LD50 = 1.05 ng/larva) followed by RH-5992 (LD50 = 8.8 ng/larva), RH-0345 (LD50 = 41.0 ng/larva), and RH-5849 (LD50 = 236.9 ng/larva). The relative potencies of these compounds to induce the transcription factor Choristoneura hormone receptor 3 (CHR3) was studied. Detectable levels of CHR3 mRNA were induced by RH-2485 at a concentration as low as 1.5 ng/larva, but similar inductions required concentrations as high as 7.5 ng of RH-5992/larva, 37.5 of RH-0345/larva, and 187.5 of RH-5849/larva. The order of potency of these four ecdysone agonists for inducing CHR3 mRNA is the same as their larval toxicity.",

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note = "Funding Information: We thank Rohm and Haas company scientists for supplying compounds, Dr. Sardar S. Sohi for critical comments on the manuscript, Ms. K. Jamieson for editorial help. This research was supported by a Canadian Forest Service/Natural Sciences and Engineering Research Council Research Partnership Program Award CRD No. 0192158, with Rohm and Haas Company.",

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AU - Sundaram, M.

AU - Palli, S. R.

AU - Ishaaya, I.

AU - Krell, P. J.

AU - Retnakaran, A.

N1 - Funding Information: We thank Rohm and Haas company scientists for supplying compounds, Dr. Sardar S. Sohi for critical comments on the manuscript, Ms. K. Jamieson for editorial help. This research was supported by a Canadian Forest Service/Natural Sciences and Engineering Research Council Research Partnership Program Award CRD No. 0192158, with Rohm and Haas Company.

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N2 - Four ecdysone agonists, RH-5849, RH-5992, RH-0345, and RH-2485, were tested for toxicity against fifth-instar larvae of Choristoneura fumiferana. RH-2485 was the most toxic (LD50 = 1.05 ng/larva) followed by RH-5992 (LD50 = 8.8 ng/larva), RH-0345 (LD50 = 41.0 ng/larva), and RH-5849 (LD50 = 236.9 ng/larva). The relative potencies of these compounds to induce the transcription factor Choristoneura hormone receptor 3 (CHR3) was studied. Detectable levels of CHR3 mRNA were induced by RH-2485 at a concentration as low as 1.5 ng/larva, but similar inductions required concentrations as high as 7.5 ng of RH-5992/larva, 37.5 of RH-0345/larva, and 187.5 of RH-5849/larva. The order of potency of these four ecdysone agonists for inducing CHR3 mRNA is the same as their larval toxicity.

AB - Four ecdysone agonists, RH-5849, RH-5992, RH-0345, and RH-2485, were tested for toxicity against fifth-instar larvae of Choristoneura fumiferana. RH-2485 was the most toxic (LD50 = 1.05 ng/larva) followed by RH-5992 (LD50 = 8.8 ng/larva), RH-0345 (LD50 = 41.0 ng/larva), and RH-5849 (LD50 = 236.9 ng/larva). The relative potencies of these compounds to induce the transcription factor Choristoneura hormone receptor 3 (CHR3) was studied. Detectable levels of CHR3 mRNA were induced by RH-2485 at a concentration as low as 1.5 ng/larva, but similar inductions required concentrations as high as 7.5 ng of RH-5992/larva, 37.5 of RH-0345/larva, and 187.5 of RH-5849/larva. The order of potency of these four ecdysone agonists for inducing CHR3 mRNA is the same as their larval toxicity.

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KW - Pest control

KW - RH-0345

KW - RH-2485

KW - RH-5849

KW - RH-5992

KW - Spruce budworm

KW - Transcription factor

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JO - Pesticide Biochemistry and Physiology

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IS - 3

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Toxicity of ecdysone agonists correlates with the induction of CHR3 mRNA in the spruce budworm

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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