Toxicogenetic study of omeprazole and the modulatory effects of retinol palmitate and ascorbic acid on Allium cepa

Antonio Lima Braga, Ag Anne Pereira Melo de Meneses, José Victor de Oliveira Santos, Antonielly Campinho dos Reis, Rosália Maria Tôrres de Lima, Ana Maria Oliveira Ferreira da Mata, Márcia Fernanda Correia Jardim Paz, Leane Brunelle dos Santos Alves, Subrata Shaw, Shaikh Jamal Uddin, Razina Rouf, Asish Kumar Das, Shrabanti Dev, Manik Chandra Shil, Jamil A. Shilpi, Ishaq N. Khan, Muhammad Torequl Islam, Eunüs S. Ali, Mohammad S. Mubarak, Siddhartha Kumar MishraJoão Marcelo de Castro e Sousa, Ana Amélia de Carvalho Melo-Cavalcante

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Omeprazole (OME) is a proton pump inhibitor used for the treatment of various gastric and intestinal disease; however, studies on its effects on the genetic materials are still restricted. The present study aimed to evaluate possible toxicogenic effects of OME in Allium cepa meristems with the application of cytogenetic biomarkers for DNA damage, mutagenic, toxic and cytotoxic effects. Additionally, retinol palmitate (RP) and ascorbic acid (AA) were also co-treated with OME to evaluate possible modulatory effects of OME-induced cytogenetic damages. OME was tested at 10, 20 and 40 μg/mL, while RP and AA at 55 μg/mL and 352.2 μg/mL, respectively. Copper sulphate (0.6 μg/mL) and dechlorinated water were used as positive control and negative control, respectively. The results suggest that OME induced genotoxicity and mutagenicity in A. cepa at all tested concentrations. It was noted that cotreatment of OME with the antioxidant vitamins RP and/or AA significantly (p < 0.05) inhibited and/or modulated all toxicogenic damages induced by OME. These observations demonstrate their antigenotoxic, antimutagenic, antitoxic and anticitotoxic effects in A. cepa. This study indicates that application of antioxidants may be useful tools to overcome OME-induced toxic effects.

Original languageEnglish
Pages (from-to)220-226
Number of pages7
JournalChemosphere
Volume204
DOIs
StatePublished - Aug 2018

Bibliographical note

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

  • Allium cepa
  • Antioxidants
  • Cytogenotoxicity
  • Omeprazole
  • Vitamins

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • General Chemistry
  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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