TY - JOUR
T1 - Toxicogenetic study of omeprazole and the modulatory effects of retinol palmitate and ascorbic acid on Allium cepa
AU - Braga, Antonio Lima
AU - de Meneses, Ag Anne Pereira Melo
AU - Santos, José Victor de Oliveira
AU - dos Reis, Antonielly Campinho
AU - de Lima, Rosália Maria Tôrres
AU - da Mata, Ana Maria Oliveira Ferreira
AU - Paz, Márcia Fernanda Correia Jardim
AU - Alves, Leane Brunelle dos Santos
AU - Shaw, Subrata
AU - Uddin, Shaikh Jamal
AU - Rouf, Razina
AU - Das, Asish Kumar
AU - Dev, Shrabanti
AU - Shil, Manik Chandra
AU - Shilpi, Jamil A.
AU - Khan, Ishaq N.
AU - Islam, Muhammad Torequl
AU - Ali, Eunüs S.
AU - Mubarak, Mohammad S.
AU - Mishra, Siddhartha Kumar
AU - e Sousa, João Marcelo de Castro
AU - Melo-Cavalcante, Ana Amélia de Carvalho
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/8
Y1 - 2018/8
N2 - Omeprazole (OME) is a proton pump inhibitor used for the treatment of various gastric and intestinal disease; however, studies on its effects on the genetic materials are still restricted. The present study aimed to evaluate possible toxicogenic effects of OME in Allium cepa meristems with the application of cytogenetic biomarkers for DNA damage, mutagenic, toxic and cytotoxic effects. Additionally, retinol palmitate (RP) and ascorbic acid (AA) were also co-treated with OME to evaluate possible modulatory effects of OME-induced cytogenetic damages. OME was tested at 10, 20 and 40 μg/mL, while RP and AA at 55 μg/mL and 352.2 μg/mL, respectively. Copper sulphate (0.6 μg/mL) and dechlorinated water were used as positive control and negative control, respectively. The results suggest that OME induced genotoxicity and mutagenicity in A. cepa at all tested concentrations. It was noted that cotreatment of OME with the antioxidant vitamins RP and/or AA significantly (p < 0.05) inhibited and/or modulated all toxicogenic damages induced by OME. These observations demonstrate their antigenotoxic, antimutagenic, antitoxic and anticitotoxic effects in A. cepa. This study indicates that application of antioxidants may be useful tools to overcome OME-induced toxic effects.
AB - Omeprazole (OME) is a proton pump inhibitor used for the treatment of various gastric and intestinal disease; however, studies on its effects on the genetic materials are still restricted. The present study aimed to evaluate possible toxicogenic effects of OME in Allium cepa meristems with the application of cytogenetic biomarkers for DNA damage, mutagenic, toxic and cytotoxic effects. Additionally, retinol palmitate (RP) and ascorbic acid (AA) were also co-treated with OME to evaluate possible modulatory effects of OME-induced cytogenetic damages. OME was tested at 10, 20 and 40 μg/mL, while RP and AA at 55 μg/mL and 352.2 μg/mL, respectively. Copper sulphate (0.6 μg/mL) and dechlorinated water were used as positive control and negative control, respectively. The results suggest that OME induced genotoxicity and mutagenicity in A. cepa at all tested concentrations. It was noted that cotreatment of OME with the antioxidant vitamins RP and/or AA significantly (p < 0.05) inhibited and/or modulated all toxicogenic damages induced by OME. These observations demonstrate their antigenotoxic, antimutagenic, antitoxic and anticitotoxic effects in A. cepa. This study indicates that application of antioxidants may be useful tools to overcome OME-induced toxic effects.
KW - Allium cepa
KW - Antioxidants
KW - Cytogenotoxicity
KW - Omeprazole
KW - Vitamins
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U2 - 10.1016/j.chemosphere.2018.04.021
DO - 10.1016/j.chemosphere.2018.04.021
M3 - Article
C2 - 29656158
AN - SCOPUS:85047404950
SN - 0045-6535
VL - 204
SP - 220
EP - 226
JO - Chemosphere
JF - Chemosphere
ER -
