Transactivation of the parathyroid hormone promoter by specificity proteins and the nuclear factor Y complex

Alexander P. Alimov, Ok Kyong Park-Sarge, Kevin D. Sarge, Hartmut H. Malluche, Nicholas J. Koszewski

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We previously identified a highly conserved specificity protein 1 (Sp1) DNA element in mammalian PTH promoters that acted as an enhancer of gene transcription and bound Sp1 and Sp3 proteins present in parathyroid gland nuclear extracts. More recently, a nuclear factor (NF)-Y element (NF-Yprox) was also described by our group, which was located approximately 30 bp downstream from the Sp1 site in the humanPTH(hPTH) promoter and by itself acted as a weak enhancer of gene transcription. We now report that Sp proteins and NF-Y can synergistically enhance transcription of a minimal hPTH promoter construct. Positioning of the Sp1 DNA element appears to be critical for this synergism because deviations of one half of a helical turn caused an approximate 60% decrease in transactivation. Finally, examination of the bovine PTH (bPTH) promoter also revealed Sp1/NF-Y synergism, in conjunction with the identification of an analogous NF-Y binding site similarly positioned downstream from the bPTH Sp1 element. In summary, synergistic transactivation of the hPTH and bPTH promoters is observed by Sp proteins and the NF-Y complex. The conservation of this transactivation in the human and bovine promoters suggests that this may be a principle means of enhancing PTH gene transcription.

Original languageEnglish
Pages (from-to)3409-3416
Number of pages8
JournalEndocrinology
Volume146
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Endocrinology

Fingerprint

Dive into the research topics of 'Transactivation of the parathyroid hormone promoter by specificity proteins and the nuclear factor Y complex'. Together they form a unique fingerprint.

Cite this