Insecticide resistance is a global problem that presents an ongoing challenge to control insects that destroy crops, trees and transmit diseases. Dramatic progress has been made during the last decade on identification of insecticide resistance-associated genes. In one of the most common resistance mechanisms, insects acquire resistance by increasing the levels of their detoxification enzymes especially the cytochrome P450 monooxygenases (P450's). Previous studies in our laboratory showed that the pyrethroid resistance in QTC279 strain of Tribolium castaneum is achieved through constitutive overexpression of the P450 gene CYP6BQ9 by 200-fold higher in the resistant strain as compared to that in the susceptible strain. RNAi-aided knockdown in the expression of probable genes that regulate P450 gene expression in QTC279 identified cap 'n' collar C (CncC) and muscle aponeurosis fibromatosis (Maf) family transcription factors as the key regulator of these genes, CncC and Maf regulate expression of multiple genes in the CYP6BQ cluster. Studies on the promoters of these genes using reporter assays identified binding sites that mediate CncC and Maf regulation of CYP6BQ gene expression.
|Number of pages||10|
|Journal||Insect Biochemistry and Molecular Biology|
|State||Published - Oct 1 2015|
Bibliographical noteFunding Information:
This work was supported by USDA-NRI-CSREES ( 2011-04636 ). This is publication number 15-08-090 from the Kentucky Agricultural Experimental Station and published with the approval of the director. This work is supported by the National Institute of Food and Agriculture, USDA, HATCH under 2351177000.
© 2015 Elsevier Ltd.
- Insecticide resistance
- Xenobiotic transcription factors
ASJC Scopus subject areas
- Molecular Biology
- Insect Science