Transcriptome response of Lolium arundinaceum to its fungal endophyte Epichloë coenophiala

Randy D. Dinkins, Padmaja Nagabhyru, Michelle A. Graham, Deborah Boykin, Christopher L. Schardl

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Tall fescue (Lolium arundinaceum) is one of the primary forage and turf grasses in temperate regions of the world. A number of favourable characteristics of tall fescue are enhanced by its seed-transmissible fungal symbiont (endophyte) Epichloë coenophiala. Our approach was to assemble the tall fescue transcriptome, then identify differentially expressed genes (DEGs) for endophyte-symbiotic (E+) vs endophyte-free (E−) clones in leaf blades, pseudostems, crowns and roots. RNA-seq reads were used to construct a tall fescue reference transcriptome and compare gene expression profiles. Over all tissues examined, 478 DEGs were identified between the E+ and E− clones for at least one tissue (more than two-fold; P < 0.0001, 238 E+ > E− and 240 E− > E+), although no genes were differentially expressed in all four tissues. Gene ontology (GO) terms, GO:0010200 (response to chitin), GO:0002679 (respiratory burst during defence response) and GO:0035556 (intracellular signal transduction) were significantly overrepresented among 25 E− > E+ DEGs in leaf blade, and a number of other DEGs were associated with defence and abiotic response. In particular, endophyte effects on various WRKY transcription factors may have implications for symbiotic stability, endophyte distribution in the plant, or defence against pathogens.

Original languageEnglish
Pages (from-to)324-337
Number of pages14
JournalNew Phytologist
Volume213
Issue number1
DOIs
StatePublished - Jan 1 2017

Bibliographical note

Publisher Copyright:
No claim to original US government works. New Phytologist © 2016 New Phytologist Trust

Keywords

  • Epichloë coenophiala
  • Lolium arundinaceum
  • RNA-seq
  • differential expression
  • transcriptome

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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