Transcriptomic analysis of mating responses in bemisia tabaci med females

Zhijia Huo, Yating Liu, Jinjian Yang, Wen Xie, Shaoli Wang, Qingjun Wu, Xuguo Zhou, Baoping Pang, Youjun Zhang

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up-and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

Original languageEnglish
Article number308
JournalInsects
Volume11
Issue number5
DOIs
StatePublished - May 2020

Bibliographical note

Funding Information:
Funding: This research was supported by the National Natural Science Foundation of China (31871970, 31672032 and 31420103919), the China Agriculture Research System (CARS-24-C-02), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Bemisia tabaci MED
  • DEG
  • Female
  • Mating responses
  • Transcriptome

ASJC Scopus subject areas

  • Insect Science

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