Transcriptomic analysis of mating responses in bemisia tabaci med females

Zhijia Huo; Yating Liu; Jinjian Yang; Wen Xie; Shaoli Wang; Qingjun Wu; Xuguo Zhou; Baoping Pang; Youjun Zhang

doi:10.3390/insects11050308

Transcriptomic analysis of mating responses in bemisia tabaci med females

Zhijia Huo, Yating Liu, Jinjian Yang, Wen Xie, Shaoli Wang, Qingjun Wu, Xuguo Zhou, Baoping Pang, Youjun Zhang

Entomology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up-and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

Original language	English
Article number	308
Journal	Insects
Volume	11
Issue number	5
DOIs	https://doi.org/10.3390/insects11050308
State	Published - May 2020

Bibliographical note

Funding Information:
Funding: This research was supported by the National Natural Science Foundation of China (31871970, 31672032 and 31420103919), the China Agriculture Research System (CARS-24-C-02), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Bemisia tabaci MED
DEG
Female
Mating responses
Transcriptome

ASJC Scopus subject areas

Insect Science

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10.3390/insects11050308

Cite this

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title = "Transcriptomic analysis of mating responses in bemisia tabaci med females",

abstract = "Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up-and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.",

keywords = "Bemisia tabaci MED, DEG, Female, Mating responses, Transcriptome",

author = "Zhijia Huo and Yating Liu and Jinjian Yang and Wen Xie and Shaoli Wang and Qingjun Wu and Xuguo Zhou and Baoping Pang and Youjun Zhang",

note = "Funding Information: Funding: This research was supported by the National Natural Science Foundation of China (31871970, 31672032 and 31420103919), the China Agriculture Research System (CARS-24-C-02), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Huo, Zhijia

AU - Liu, Yating

AU - Yang, Jinjian

AU - Xie, Wen

AU - Wang, Shaoli

AU - Wu, Qingjun

AU - Zhou, Xuguo

AU - Pang, Baoping

AU - Zhang, Youjun

N1 - Funding Information: Funding: This research was supported by the National Natural Science Foundation of China (31871970, 31672032 and 31420103919), the China Agriculture Research System (CARS-24-C-02), the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS) and the Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/5

Y1 - 2020/5

N2 - Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up-and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

AB - Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up-and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

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Transcriptomic analysis of mating responses in bemisia tabaci med females

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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