Abstract
Studies of the spirochete Borrelia burgdorferi have been hindered by the scarcity of genetic tools that can be used in these bacteria. For the first time, a method has been developed by which heterologous DNA (DNA without a naturally occurring B. burgdorferi homolog) can be introduced into and persistently maintained by B. burgdorferi. This technique uses integration of circular DNA into the bacterial genome via a single-crossover event. The ability to transform B. burgdorferi with heterologous DNA will now permit a wide range of experiments on the biology of these bacteria and their involvement in the many facets of Lyme disease.
Original language | English |
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Pages (from-to) | 4850-4855 |
Number of pages | 6 |
Journal | Journal of Bacteriology |
Volume | 180 |
Issue number | 18 |
DOIs | |
State | Published - Sep 1998 |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology