Transformation of the lyme disease spirochete borrelia Burgdorferi with heterologous DNA

Brian Stevenson, James L. Bono, Abdallah Elias, Kit Tilly, Patricia Rosa

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Studies of the spirochete Borrelia burgdorferi have been hindered by the scarcity of genetic tools that can be used in these bacteria. For the first time, a method has been developed by which heterologous DNA (DNA without a naturally occurring B. burgdorferi homolog) can be introduced into and persistently maintained by B. burgdorferi. This technique uses integration of circular DNA into the bacterial genome via a single-crossover event. The ability to transform B. burgdorferi with heterologous DNA will now permit a wide range of experiments on the biology of these bacteria and their involvement in the many facets of Lyme disease.

Original languageEnglish
Pages (from-to)4850-4855
Number of pages6
JournalJournal of Bacteriology
Volume180
Issue number18
DOIs
StatePublished - Sep 1998

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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