A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.
|Number of pages||3|
|Journal||World Journal of Microbiology and Biotechnology|
|State||Published - 1997|
Bibliographical noteFunding Information:
This work was supported by grants from the Department of Biotechnology (Government of India) and the Rockefeller Foundation (USA).
- Benomyl resistance
- Magnaporthe grisea
- protoplast transformation
- selectable marker
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology