Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance

P. Kachroo, A. Potnis, B. B. Chattoo

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.

Original languageEnglish
Pages (from-to)185-187
Number of pages3
JournalWorld Journal of Microbiology and Biotechnology
Issue number2
StatePublished - 1997

Bibliographical note

Funding Information:
This work was supported by grants from the Department of Biotechnology (Government of India) and the Rockefeller Foundation (USA).


  • Benomyl resistance
  • Magnaporthe grisea
  • protoplast transformation
  • selectable marker

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology


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