Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance

P. Kachroo; A. Potnis; B. B. Chattoo

doi:10.1023/A:1018589714357

Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance

P. Kachroo, A. Potnis, B. B. Chattoo

Plant Pathology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.

Original language	English
Pages (from-to)	185-187
Number of pages	3
Journal	World Journal of Microbiology and Biotechnology
Volume	13
Issue number	2
DOIs	https://doi.org/10.1023/A:1018589714357
State	Published - 1997

Bibliographical note

Funding Information:
This work was supported by grants from the Department of Biotechnology (Government of India) and the Rockefeller Foundation (USA).

Funding

This work was supported by grants from the Department of Biotechnology (Government of India) and the Rockefeller Foundation (USA).

Funders	Funder number
Rockefeller Foundation, The
Department of Biotechnology, Ministry of Science and Technology, India

Keywords

Benomyl resistance
Magnaporthe grisea
protoplast transformation
selectable marker

ASJC Scopus subject areas

Biotechnology
Physiology
Applied Microbiology and Biotechnology

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title = "Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance",

abstract = "A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.",

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author = "P. Kachroo and A. Potnis and Chattoo, \{B. B.\}",

note = "Funding Information: This work was supported by grants from the Department of Biotechnology (Government of India) and the Rockefeller Foundation (USA).",

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doi = "10.1023/A:1018589714357",

language = "English",

volume = "13",

pages = "185--187",

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T1 - Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance

AU - Kachroo, P.

AU - Potnis, A.

AU - Chattoo, B. B.

N1 - Funding Information: This work was supported by grants from the Department of Biotechnology (Government of India) and the Rockefeller Foundation (USA).

PY - 1997

Y1 - 1997

N2 - A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.

AB - A transformation method based on a dominant selectable marker (benomyl resistance) was developed for the rice blast fungus Magnaporthe grisea. The heterologous gene for β-tubulin from Neurospora crassa (pBT3) was used to obtain benomyl-resistant M. grisea transformants at a frequency of 20 to 30/μg of DNA. Control transformations carried out with a plasmid conferring hygromycin resistance or a derivative of pBT3 containing a repetitive DNA sequence, yielded the same frequency of transformation as that of pBT3. Molecular analysis of the transformants indicated multiple integration of the vector DNA.

KW - Benomyl resistance

KW - Magnaporthe grisea

KW - protoplast transformation

KW - selectable marker

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Transformation of the rice blast fungus Magnaporthe grisea to benomyl resistance

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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