Transforming Growth Factor-β2: Cdna Cloning and Sequence Analysis

Linda Madisen, Nancy R. Webb, Timothy M. Rose, Hans Marquardt, Tatsuhiko Ikeda, Daniel Twardzik, Saeid Seyedin, A. F. Purchio

Research output: Contribution to journalArticlepeer-review

192 Scopus citations


We have obtained a cDNA clone coding for human transforming growth factor (TGF)-β2. The clone was isolated from a tamoxifen-treated human prostatic adenocarcinoma cell line (PC-3) using oligonucleotide probes based on the partial amino acid sequence of purified TGF-β2. The cDNA sequence predicts that TGF-β2 is synthesized as a 442-amino-acid polypeptide precursor from which the mature 112-amino-acid TGF-β2 subunit is derived by proteolytic cleavage. The proteins coded for by the human TGF-β1 and TGF-β2 cDNAs show an overall homology of 41%. The mature and amino-terminal precursor regions show 71% and 31% homology, respectively. Northern blot analysis identified TGF-β2 transcripts of 4.1, 5.1, and 6.5 kb using mRNA from several different sources. Analysis of polyadenylated RNA from tamoxifen-treated PC-3 cells showed that these cells contain higher numbers of transcripts for TGF-β1 than for TGF-β2, although they produce more TGF-β2 protein than TGF-β1. This suggests that there is a post-transcriptional level of regulation for the production of these proteins.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
Issue number1
StatePublished - Jan 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics


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