Transforming growth factor-beta inhibits human vascular smooth muscle cell growth and migration

S. Mii, J. A. Ware, K. C. Kent, L. M. Graham, M. Davies, D. Minion

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Background. Transforming growth factor-β (TGF-β) is released after vascular injury and may influence the healing response of the vessel wall. We investigated the effect of this growth factor on proliferation and migration of human venous smooth muscle cells (SMC) and the signal transduction mechanisms through which TGF-β exerts this effect. Methods. SMC derived from human saphenous vein were used in a 72-hour proliferation assay and a 6-day migration assay. Cells were exposed to TGF-β alone and in the presence of platelet-derived growth factor (PDGF), basic fibroblast growth factor (b- FGF), epidermal growth factor, and serum. The ability of TGF-β to activate tyrosine kinases, phosphatases, or protein kinase C was evaluated by use of Western blotting with an antiphosphotyrosine antibody. Results. In a concentration-dependent manner, TGF-β inhibited proliferation induced by PDGF, b-FGF, epidermal growth factor, and serum. This inhibitory effect was independent of SMC density. TGF-β also inhibited migration induced by PDGF and b-FGF. Exposure of cells to TGF-β did not lead to tyrosine phosphorylation of cellular substrates or activation of protein kinase C. Conclusions. TGF-β inhibits both migration and proliferation of human SMC. This inhibitory effect is not mediated through protein kinase C, mitogen- activated protein kinase, or tyrosine kinases.

Original languageEnglish
Pages (from-to)464-470
Number of pages7
JournalSurgery
Volume114
Issue number2
StatePublished - 1993

ASJC Scopus subject areas

  • Surgery

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