Transgenic soya bean seeds accumulating β-carotene exhibit the collateral enhancements of oleate and protein content traits

Monica A. Schmidt, Wayne A. Parrott, David F. Hildebrand, R. Howard Berg, Amanda Cooksey, Ken Pendarvis, Yonghua He, Fiona Mccarthy, Eliot M. Herman

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Summary: Transgenic soya bean (Glycine max) plants overexpressing a seed-specific bacterial phytoene synthase gene from Pantoea ananatis modified to target to plastids accumulated 845 μg β carotene g-1 dry seed weight with a desirable 12:1 ratio of β to α. The β carotene accumulating seeds exhibited a shift in oil composition increasing oleic acid with a concomitant decrease in linoleic acid and an increase in seed protein content by at least 4% (w/w). Elevated β-carotene accumulating soya bean cotyledons contain 40% the amount of abscisic acid compared to nontransgenic cotyledons. Proteomic and nontargeted metabolomic analysis of the mid-maturation β-carotene cotyledons compared to the nontransgenic did not reveal any significant differences that would account for the altered phenotypes of both elevated oleate and protein content. Transcriptomic analysis, confirmed by RT-PCR, revealed a number of significant differences in ABA-responsive transcripton factor gene expression in the crtB transgenics compared to nontransgenic cotyledons of the same maturation stage. The altered seed composition traits seem to be attributed to altered ABA hormone levels varying transcription factor expression. The elevated β-carotene, oleic acid and protein traits in the β-carotene soya beans confer a substantial additive nutritional quality to soya beans.

Original languageEnglish
Pages (from-to)590-600
Number of pages11
JournalPlant Biotechnology Journal
Issue number4
StatePublished - May 1 2015

Bibliographical note

Publisher Copyright:
© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.


  • Carotenoid
  • Elevated protein
  • Oleic acid
  • Seed
  • Soybean

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science


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