An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein–protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes.
|Number of pages||7|
|Journal||Methods in Molecular Biology|
|State||Published - 2015|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media New York 2015.
- Agroinfi ltration
- Polyadenylation sites
- Protein–protein interactions
- Transient expression
ASJC Scopus subject areas
- Molecular Biology