Transient expression using agroinfi ltration to study polyadenylation in plants

Carol Von Lanken, Arthur G. Hunt

Research output: Contribution to journalArticlepeer-review


An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein–protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalMethods in Molecular Biology
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.


  • Agroinfi ltration
  • Polyadenylation sites
  • Protein–protein interactions
  • Transient expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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