Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Hiroko P. Indo; Hiromu Ito; Keiichi Nakagawa; Luksana Chaiswing; Hideyuki J. Majima

doi:10.1016/j.abb.2021.108853

Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Hiroko P. Indo, Hiromu Ito, Keiichi Nakagawa, Luksana Chaiswing, Hideyuki J. Majima

Toxicology and Cancer Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

Original language	English
Article number	108853
Journal	Archives of Biochemistry and Biophysics
Volume	703
DOIs	https://doi.org/10.1016/j.abb.2021.108853
State	Published - May 30 2021

Bibliographical note

Funding Information:
The HSP47 vector was a kind gift from Professor Kazuhiro Nagata of Kyoto Sangyo University. The authors would like to express sincere appreciation for the gift. The authors gratefully thank Drs Shosei Kishida and Shigeaki Suenaga of Kagoshima University, Drs. Noriaki Ishioka and Sachiko Yano of Japan Aerospace Exploration Agency (JAXA), and Drs. Daret K St. Clair and William St. Clair of University of Kentucky for encouragement to complete this study. We thank Mr. Shigeki Saegusa of the University of Tokyo Hospital for his help and guidance on electron irradiation.

Publisher Copyright:
© 2021 Elsevier Inc.

Keywords

Electron and X-ray irradiation
Farnesylation
HSP47
Mitochondria
Myristoylation
Reactive oxygen species

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.abb.2021.108853

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title = "Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation",

abstract = "Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.",

keywords = "Electron and X-ray irradiation, Farnesylation, HSP47, Mitochondria, Myristoylation, Reactive oxygen species",

author = "Indo, {Hiroko P.} and Hiromu Ito and Keiichi Nakagawa and Luksana Chaiswing and Majima, {Hideyuki J.}",

note = "Funding Information: The HSP47 vector was a kind gift from Professor Kazuhiro Nagata of Kyoto Sangyo University. The authors would like to express sincere appreciation for the gift. The authors gratefully thank Drs Shosei Kishida and Shigeaki Suenaga of Kagoshima University, Drs. Noriaki Ishioka and Sachiko Yano of Japan Aerospace Exploration Agency (JAXA), and Drs. Daret K St. Clair and William St. Clair of University of Kentucky for encouragement to complete this study. We thank Mr. Shigeki Saegusa of the University of Tokyo Hospital for his help and guidance on electron irradiation. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

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AU - Indo, Hiroko P.

AU - Ito, Hiromu

AU - Nakagawa, Keiichi

AU - Chaiswing, Luksana

AU - Majima, Hideyuki J.

N1 - Funding Information: The HSP47 vector was a kind gift from Professor Kazuhiro Nagata of Kyoto Sangyo University. The authors would like to express sincere appreciation for the gift. The authors gratefully thank Drs Shosei Kishida and Shigeaki Suenaga of Kagoshima University, Drs. Noriaki Ishioka and Sachiko Yano of Japan Aerospace Exploration Agency (JAXA), and Drs. Daret K St. Clair and William St. Clair of University of Kentucky for encouragement to complete this study. We thank Mr. Shigeki Saegusa of the University of Tokyo Hospital for his help and guidance on electron irradiation. Publisher Copyright: © 2021 Elsevier Inc.

PY - 2021/5/30

Y1 - 2021/5/30

N2 - Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

AB - Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.

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KW - Farnesylation

KW - HSP47

KW - Mitochondria

KW - Myristoylation

KW - Reactive oxygen species

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Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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