Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: A long-term evaluation

Luciana S. Branco-de-Almeida; Yenisel Cruz-Almeida; Yandy Gonzalez-Marrero; Rachad Kudsi; Izabel C.V. de Oliveira; Bogdan Dolia; Hong Huang; Ikramuddin Aukhil; Peter Harrison; Luciana M. Shaddox

doi:10.1111/jcpe.13404

Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: A long-term evaluation

Luciana S. Branco-de-Almeida, Yenisel Cruz-Almeida, Yandy Gonzalez-Marrero, Rachad Kudsi, Izabel C.V. de Oliveira, Bogdan Dolia, Hong Huang, Ikramuddin Aukhil, Peter Harrison, Luciana M. Shaddox

College of Dentistry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Aim: To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. Methods: Sixty-six African-Americans with LAP (7–21 years old) were included. After periodontal examination, all patients received periodontal treatment with mechanical debridement plus systemic amoxicillin/metronidazole for 7 days. Gingival crevicular fluid was collected from diseased and healthy sites at baseline and 3, 6, 12, and 24 months following treatment. Levels of 16 inflammatory/bone resorption markers were determined using Milliplex^®. Univariate and correlation analyses were performed among all parameters/biomarkers. Discriminant analyses (DA) evaluated profile differences between LAP diseased and healthy sites at each time point as compared to the baseline. Results: Reductions in the clinical parameters (except for visible plaque) were observed at all time points compared to the baseline. Levels of IL-12p70, IL-2, IL-6, MIP-1α, RANKL, and OPG were reduced after treatment, and several cytokines/chemokines were correlated with clinical parameters reductions. DA showed that differences in the immunoinflammatory profiles between LAP diseased and healthy sites decreased after periodontal treatment compared to the baseline. Conclusions: Periodontal treatment modified the local immunoinflammatory profile of LAP sites in the long term, as suggested by changes in biomarkers from baseline, along with clinical stability of the disease. (Clinicaltrials.gov number, NCT01330719).

Original language	English
Pages (from-to)	237-248
Number of pages	12
Journal	Journal of Clinical Periodontology
Volume	48
Issue number	2
DOIs	https://doi.org/10.1111/jcpe.13404
State	Published - Feb 2021

Bibliographical note

Publisher Copyright:
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Funding

We would like to thank the National Institutes of Health/ National Institute of Dental and Craniofacial Research for the financial support/fellowship (NIH/NIDCR; grants #R01DE019456 and #R90DE22530‐4). The authors are also grateful to dentists and all the staff from the health departments of Leon County, Duval County, and from the University of Florida College of Dentistry (Florida, USA). The authors declare that there are no conflicts of interest with respect to the authorship and/or publication of this article. We would like to thank the National Institutes of Health/ National Institute of Dental and Craniofacial Research for the financial support/fellowship (NIH/NIDCR; grants #R01DE019456 and #R90DE22530-4). The authors are also grateful to dentists and all the staff from the health departments of Leon County, Duval County, and from the University of Florida College of Dentistry (Florida, USA). The authors declare that there are no conflicts of interest with respect to the authorship and/or publication of this article.

Funders	Funder number
NIH/NIDCR	90DE22530‐4
University of Florida College of Dentistry
National Institutes of Health (NIH)
National Institute of Dental and Craniofacial Research	R90DE022530, 01DE019456, 90DE22530-4

Keywords

aggressive periodontitis
gingival crevicular fluid
inflammation
long-term effects
therapy

ASJC Scopus subject areas

Periodontics

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10.1111/jcpe.13404

Cite this

Branco-de-Almeida, L. S., Cruz-Almeida, Y., Gonzalez-Marrero, Y., Kudsi, R., de Oliveira, I. C. V., Dolia, B., Huang, H., Aukhil, I., Harrison, P., & Shaddox, L. M. (2021). Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: A long-term evaluation. Journal of Clinical Periodontology, 48(2), 237-248. https://doi.org/10.1111/jcpe.13404

@article{d21166ef16fa466b8d3b8fd43c4ea670,

title = "Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: A long-term evaluation",

abstract = "Aim: To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. Methods: Sixty-six African-Americans with LAP (7–21 years old) were included. After periodontal examination, all patients received periodontal treatment with mechanical debridement plus systemic amoxicillin/metronidazole for 7 days. Gingival crevicular fluid was collected from diseased and healthy sites at baseline and 3, 6, 12, and 24 months following treatment. Levels of 16 inflammatory/bone resorption markers were determined using Milliplex{\textregistered}. Univariate and correlation analyses were performed among all parameters/biomarkers. Discriminant analyses (DA) evaluated profile differences between LAP diseased and healthy sites at each time point as compared to the baseline. Results: Reductions in the clinical parameters (except for visible plaque) were observed at all time points compared to the baseline. Levels of IL-12p70, IL-2, IL-6, MIP-1α, RANKL, and OPG were reduced after treatment, and several cytokines/chemokines were correlated with clinical parameters reductions. DA showed that differences in the immunoinflammatory profiles between LAP diseased and healthy sites decreased after periodontal treatment compared to the baseline. Conclusions: Periodontal treatment modified the local immunoinflammatory profile of LAP sites in the long term, as suggested by changes in biomarkers from baseline, along with clinical stability of the disease. (Clinicaltrials.gov number, NCT01330719).",

keywords = "aggressive periodontitis, gingival crevicular fluid, inflammation, long-term effects, therapy",

author = "Branco-de-Almeida, \{Luciana S.\} and Yenisel Cruz-Almeida and Yandy Gonzalez-Marrero and Rachad Kudsi and \{de Oliveira\}, \{Izabel C.V.\} and Bogdan Dolia and Hong Huang and Ikramuddin Aukhil and Peter Harrison and Shaddox, \{Luciana M.\}",

note = "Publisher Copyright: {\textcopyright} 2020 John Wiley \& Sons A/S. Published by John Wiley \& Sons Ltd",

year = "2021",

month = feb,

doi = "10.1111/jcpe.13404",

language = "English",

volume = "48",

pages = "237--248",

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Branco-de-Almeida, LS, Cruz-Almeida, Y, Gonzalez-Marrero, Y, Kudsi, R, de Oliveira, ICV, Dolia, B, Huang, H, Aukhil, I, Harrison, P & Shaddox, LM 2021, 'Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: A long-term evaluation', Journal of Clinical Periodontology, vol. 48, no. 2, pp. 237-248. https://doi.org/10.1111/jcpe.13404

TY - JOUR

T1 - Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles

T2 - A long-term evaluation

AU - Branco-de-Almeida, Luciana S.

AU - Cruz-Almeida, Yenisel

AU - Gonzalez-Marrero, Yandy

AU - Kudsi, Rachad

AU - de Oliveira, Izabel C.V.

AU - Dolia, Bogdan

AU - Huang, Hong

AU - Aukhil, Ikramuddin

AU - Harrison, Peter

AU - Shaddox, Luciana M.

PY - 2021/2

Y1 - 2021/2

N2 - Aim: To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. Methods: Sixty-six African-Americans with LAP (7–21 years old) were included. After periodontal examination, all patients received periodontal treatment with mechanical debridement plus systemic amoxicillin/metronidazole for 7 days. Gingival crevicular fluid was collected from diseased and healthy sites at baseline and 3, 6, 12, and 24 months following treatment. Levels of 16 inflammatory/bone resorption markers were determined using Milliplex®. Univariate and correlation analyses were performed among all parameters/biomarkers. Discriminant analyses (DA) evaluated profile differences between LAP diseased and healthy sites at each time point as compared to the baseline. Results: Reductions in the clinical parameters (except for visible plaque) were observed at all time points compared to the baseline. Levels of IL-12p70, IL-2, IL-6, MIP-1α, RANKL, and OPG were reduced after treatment, and several cytokines/chemokines were correlated with clinical parameters reductions. DA showed that differences in the immunoinflammatory profiles between LAP diseased and healthy sites decreased after periodontal treatment compared to the baseline. Conclusions: Periodontal treatment modified the local immunoinflammatory profile of LAP sites in the long term, as suggested by changes in biomarkers from baseline, along with clinical stability of the disease. (Clinicaltrials.gov number, NCT01330719).

AB - Aim: To evaluate the local immunoinflammatory profiles in localized aggressive periodontitis patients (LAP) before and after periodontal treatment and maintenance. Methods: Sixty-six African-Americans with LAP (7–21 years old) were included. After periodontal examination, all patients received periodontal treatment with mechanical debridement plus systemic amoxicillin/metronidazole for 7 days. Gingival crevicular fluid was collected from diseased and healthy sites at baseline and 3, 6, 12, and 24 months following treatment. Levels of 16 inflammatory/bone resorption markers were determined using Milliplex®. Univariate and correlation analyses were performed among all parameters/biomarkers. Discriminant analyses (DA) evaluated profile differences between LAP diseased and healthy sites at each time point as compared to the baseline. Results: Reductions in the clinical parameters (except for visible plaque) were observed at all time points compared to the baseline. Levels of IL-12p70, IL-2, IL-6, MIP-1α, RANKL, and OPG were reduced after treatment, and several cytokines/chemokines were correlated with clinical parameters reductions. DA showed that differences in the immunoinflammatory profiles between LAP diseased and healthy sites decreased after periodontal treatment compared to the baseline. Conclusions: Periodontal treatment modified the local immunoinflammatory profile of LAP sites in the long term, as suggested by changes in biomarkers from baseline, along with clinical stability of the disease. (Clinicaltrials.gov number, NCT01330719).

KW - aggressive periodontitis

KW - gingival crevicular fluid

KW - inflammation

KW - long-term effects

KW - therapy

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DO - 10.1111/jcpe.13404

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Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: A long-term evaluation

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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