TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells

Dhanendra Tomar; Rochika Singh; Arun Kumar Singh; Chirayu D. Pandya; Rajesh Singh

doi:10.1016/j.bbamcr.2011.11.015

TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells

Dhanendra Tomar, Rochika Singh, Arun Kumar Singh, Chirayu D. Pandya, Rajesh Singh

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

Autophagy is one of the cellular adaptive processes that provide protection against many pathological conditions like infection, cancer, neurodegeneration, and aging. Recent evidences suggest that ubiquitination plays an important role in degradation of proteins or defective organelle either through proteasome or autophagy. In this study, we describe the role of TRIM13, ER resident ubiquitin E3 ligase in induction of autophagy and its role during ER stress. The ectopic expression of TRIM13 in HEK-293 cells induces autophagy. Domain mapping showed that coiled-coil (CC) domain is required for induction of autophagy. TRIM13 is stabilized during ER stress, interacts with p62/SQSTM1 and co-localizes with DFCP1. TRIM13 regulates initiation of autophagy during ER stress and decreases the clonogenic ability of the cells. This study for the first time demonstrates the role of TRIM13 in induction of autophagy which may play an important role in regulation of ER stress and may act as tumor suppressor.

Original language	English
Pages (from-to)	316-326
Number of pages	11
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1823
Issue number	2
DOIs	https://doi.org/10.1016/j.bbamcr.2011.11.015
State	Published - Feb 2012

Bibliographical note

Funding Information:
This study was supported by the Program Support to Indian Institute of Advanced Research (IIAR) sponsored by the Department of Biotechnology, Government of India (grant to RS) and grant from the Puri Foundation for Education in India (grant to RS). Dhanendra Tomar received Junior Research Fellowship from the Council of Scientific and Industrial Research (CSIR), Government of India.

Keywords

Autophagy
ER stress
TRIM
Ubiquitin E3 ligase
Ubiquitin proteasome system

ASJC Scopus subject areas

Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbamcr.2011.11.015

Cite this

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abstract = "Autophagy is one of the cellular adaptive processes that provide protection against many pathological conditions like infection, cancer, neurodegeneration, and aging. Recent evidences suggest that ubiquitination plays an important role in degradation of proteins or defective organelle either through proteasome or autophagy. In this study, we describe the role of TRIM13, ER resident ubiquitin E3 ligase in induction of autophagy and its role during ER stress. The ectopic expression of TRIM13 in HEK-293 cells induces autophagy. Domain mapping showed that coiled-coil (CC) domain is required for induction of autophagy. TRIM13 is stabilized during ER stress, interacts with p62/SQSTM1 and co-localizes with DFCP1. TRIM13 regulates initiation of autophagy during ER stress and decreases the clonogenic ability of the cells. This study for the first time demonstrates the role of TRIM13 in induction of autophagy which may play an important role in regulation of ER stress and may act as tumor suppressor.",

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author = "Dhanendra Tomar and Rochika Singh and Singh, {Arun Kumar} and Pandya, {Chirayu D.} and Rajesh Singh",

note = "Funding Information: This study was supported by the Program Support to Indian Institute of Advanced Research (IIAR) sponsored by the Department of Biotechnology, Government of India (grant to RS) and grant from the Puri Foundation for Education in India (grant to RS). Dhanendra Tomar received Junior Research Fellowship from the Council of Scientific and Industrial Research (CSIR), Government of India. ",

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AU - Singh, Rajesh

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AB - Autophagy is one of the cellular adaptive processes that provide protection against many pathological conditions like infection, cancer, neurodegeneration, and aging. Recent evidences suggest that ubiquitination plays an important role in degradation of proteins or defective organelle either through proteasome or autophagy. In this study, we describe the role of TRIM13, ER resident ubiquitin E3 ligase in induction of autophagy and its role during ER stress. The ectopic expression of TRIM13 in HEK-293 cells induces autophagy. Domain mapping showed that coiled-coil (CC) domain is required for induction of autophagy. TRIM13 is stabilized during ER stress, interacts with p62/SQSTM1 and co-localizes with DFCP1. TRIM13 regulates initiation of autophagy during ER stress and decreases the clonogenic ability of the cells. This study for the first time demonstrates the role of TRIM13 in induction of autophagy which may play an important role in regulation of ER stress and may act as tumor suppressor.

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KW - Ubiquitin proteasome system

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TRIM13 regulates ER stress induced autophagy and clonogenic ability of the cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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