Triticuside a, a dietary flavonoid, inhibits proliferation of human breast cancer cells via inducing apoptosis

Yu Shan, Yan Cheng, Yi Zhang, Fu Qin Guan, Hao Sun, Xing Cong Ren, Yu Chen, Xu Feng, Jin Ming Yang

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

In this study we demonstrated that Triticuside A, one of the flavonoid compounds isolated from wheat bran, induced apoptosis and inhibited proliferation of human breast cancer cells. Triticuside A inhibited the proliferation of human breast cancer cells (MCF-7 and MDA-MB-231) in a dose-dependent manner but barely showed cytotoxicity to the normal human fibroblasts. Triticuside A-induced apoptosis was accompanied by a significant decrease of Mcl-1 and Bcl-2 proteins and by an increase of cleavage of caspases-3, -7, -9, and PARP. Triticuside A also suppressed the level of phospho-Akt and its downstream targets, mTOR and P70 S6 kinase. LY294002, a specific inhibitor of PI3K, significantly enhanced the Triticuside A-induced apoptosis. Moreover LY294002 not only downregulated the level of phospho-Akt but also enhanced the inhibition of Mcl-1 expression when combined with Triticuside A. Our results demonstrate for the first time the specific apoptogenic activity of Triticuside A in tumor cells and involvement of the mitochondrial apoptosis pathway and Akt/mTOR signaling pathway. Thus, Triticuside A may be a potentially useful wheat bran component that can be used for prevention or treatment of breast cancer.

Original languageEnglish
Pages (from-to)891-899
Number of pages9
JournalNutrition and Cancer
Volume65
Issue number6
DOIs
StatePublished - Aug 1 2013

Bibliographical note

Funding Information:
This work was supported by grants from the National Natural Science Foundation of China (31100251) and Jiangsu Government Scholarship for Overseas Studies.

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

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