TY - JOUR
T1 - Tritium (3H) radiolabeling of protein A and antibody to high specific activity
T2 - Application to cell surface antigen radioimmunoassays
AU - Wilder, Ronald L.
AU - Yuen, Catherine C.
AU - Subbarao, Bondada
AU - Woods, Virgil L.
AU - Alexander, Cornelius B.
AU - Mage, Rose G.
PY - 1979/7/24
Y1 - 1979/7/24
N2 - Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (>106 cpm/μg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.
AB - Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (>106 cpm/μg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.
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U2 - 10.1016/0022-1759(79)90192-3
DO - 10.1016/0022-1759(79)90192-3
M3 - Article
C2 - 479606
AN - SCOPUS:0018657407
SN - 0022-1759
VL - 28
SP - 255
EP - 266
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 3-4
ER -