Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (>106 cpm/μg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.
|Number of pages||12|
|Journal||Journal of Immunological Methods|
|State||Published - Jul 24 1979|
ASJC Scopus subject areas
- Immunology and Allergy