TY - JOUR
T1 - Tumor necrosis factor reduces proteoglycan synthesis in cultured endothelial cells
AU - Ramasamy, S.
AU - Lipke, D. W.
AU - McClain, C. J.
AU - Hennig, B.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1995/1
Y1 - 1995/1
N2 - Tumor necrosis factor (TNF)‐induced disruption of vascular endothelial barrier function may be due in part to alterations in proteoglycan metabolism. To test this hypothesis, confluent endothelial cell monolayers were exposed for 24 h to 500 or 1,000 U of TNF per mililiter of culture medium together with 20 μCi Na235SO4. HPLC anion‐exchange separation of proteoglycans secreted into media of control as well as TNF‐treated cultures revealed one major peak (representing 95% of total radioactivity) and one minor peak (representing 5% of total radioactivity), which eluted at 0.6 and 0.9 M NaCl, respectively. One single peak was obtained from control as well as TNF‐treated endothelial cell monolayers and eluted at 1.2 M NaCl. TNF treatment did not change the total quantity of radioactive proteoglycans secreted into the media but significantly decreased the amount of proteoglycans in endothelial cell monolayers. However, TNF treatment did not alter the size or glycosaminoglycan (GAG) composition of the proteoglycans either in the media or in the cell monolayers. In addition, mRNA levels of specific proteoglycans, perlecan and biglycan, were measured upon TNF treatment, using Northern analysis. TNF treatment caused a dose‐dependent decrease in mRNA levels for the biglycan in endothelial cultures. These results suggest that TNF decreases production of proteoglycans and alters normal endothelial cell proteoglycan metabolism which may be sufficient to impair endothelial barrier function. © 1995 Wiley‐Liss, Inc.
AB - Tumor necrosis factor (TNF)‐induced disruption of vascular endothelial barrier function may be due in part to alterations in proteoglycan metabolism. To test this hypothesis, confluent endothelial cell monolayers were exposed for 24 h to 500 or 1,000 U of TNF per mililiter of culture medium together with 20 μCi Na235SO4. HPLC anion‐exchange separation of proteoglycans secreted into media of control as well as TNF‐treated cultures revealed one major peak (representing 95% of total radioactivity) and one minor peak (representing 5% of total radioactivity), which eluted at 0.6 and 0.9 M NaCl, respectively. One single peak was obtained from control as well as TNF‐treated endothelial cell monolayers and eluted at 1.2 M NaCl. TNF treatment did not change the total quantity of radioactive proteoglycans secreted into the media but significantly decreased the amount of proteoglycans in endothelial cell monolayers. However, TNF treatment did not alter the size or glycosaminoglycan (GAG) composition of the proteoglycans either in the media or in the cell monolayers. In addition, mRNA levels of specific proteoglycans, perlecan and biglycan, were measured upon TNF treatment, using Northern analysis. TNF treatment caused a dose‐dependent decrease in mRNA levels for the biglycan in endothelial cultures. These results suggest that TNF decreases production of proteoglycans and alters normal endothelial cell proteoglycan metabolism which may be sufficient to impair endothelial barrier function. © 1995 Wiley‐Liss, Inc.
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U2 - 10.1002/jcp.1041620114
DO - 10.1002/jcp.1041620114
M3 - Article
C2 - 7814444
AN - SCOPUS:0028899599
SN - 0021-9541
VL - 162
SP - 119
EP - 126
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 1
ER -