Two different repressors collaborate to restrict expression of the yeast glucose transporter genes HXT2 and HXT4 to low levels of glucose

Sabire Ozcan, Mark Johnston

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Transcription of the yeast and HXT4 genes, which encode glucose transporters, is induced only by low levels of glucose. This low-glucose- induced expression is mediated by two independent repression mechanisms: in the absence of glucose, transcription of both genes is prevented by Rgt1p, a C6 zinc cluster protein; at high levels of glucose, expression of HXT2 and HXT4 is repressed by Mig1p. Only at low glucose concentrations are both repressors inactive, leading to a 10- to 20-fold induction of gene expression. Mig1p and Rgt1p act directly on HXT2 and HXT4 by binding to their promoters. This transcriptional regulation is physiologically very important to the yeast cell because it causes these glucose transporters to be expressed only in low-glucose media, in which they are required for growth.

Original languageEnglish
Pages (from-to)5536-5545
Number of pages10
JournalMolecular and Cellular Biology
Volume16
Issue number10
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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