Two fep genes are required for ferrienterochelin uptake in Escherichia coli K-12

J. R. Pierce, C. L. Pickett, C. F. Earhart

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Escherichia coli mutants defective in the as similation of iron from ferrienterochelin were isolated and characterized. One mutant was able to bind ferrienterochelin to its outer membrane but could not transport it into the cell. Complementation tests with λ hybrid phage were employed to distinguish the defective gene, which we term fepB, from fepA, the structural gene for the outer membrane ferrienterochelin receptor protein. These same physiological and genetic tests were employed to tentatively classify several previously described fep mutants as carrying either fepA or fepB. The data demonstrate the existence of fepB and provide an explanation for previous difficulties in identifying fepB mutants.

Original languageEnglish
Pages (from-to)330-336
Number of pages7
JournalJournal of Bacteriology
Volume155
Issue number1
StatePublished - 1983

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Two fep genes are required for ferrienterochelin uptake in Escherichia coli K-12'. Together they form a unique fingerprint.

Cite this