Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

Dokyoung Kim; Jinyoung Kang; Taejun Wang; Hye Gun Ryu; Jonathan M. Zuidema; Jinmyoung Joo; Muwoong Kim; Youngbuhm Huh; Junyang Jung; Kyo Han Ahn; Ki Hean Kim; Michael J. Sailor

doi:10.1002/adma.201703309

Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

Dokyoung Kim, Jinyoung Kang, Taejun Wang, Hye Gun Ryu, Jonathan M. Zuidema, Jinmyoung Joo, Muwoong Kim, Youngbuhm Huh, Junyang Jung, Kyo Han Ahn, Ki Hean Kim, Michael J. Sailor

Mechanical and Aerospace Engineering

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650–1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.

Original language	English
Article number	1703309
Journal	Advanced Materials
Volume	29
Issue number	39
DOIs	https://doi.org/10.1002/adma.201703309
State	Published - Oct 18 2017

Bibliographical note

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Funding

D.K., J.K., and T.W. contributed equally to this work. This work was supported by the National Science Foundation under Grant No. CBET-1603177 and by the Defense Advanced Research Projects Agency (DARPA) under Cooperative Agreement HR0011-13-2-0017. The content of the information within this document does not necessarily reflect the position or the policy of the Government. D.K. and J.K. acknowledge financial support from the UCSD Frontiers of Innovation Scholars Program (FISP) fellowship. D.K. thanks the Basic Science Research Program of the Korea National Research Foundation (NRF) funded by the Ministry of Education (Grant No. 2016R1A6A3A03006343). K.H.A. thanks the Korea NRF (Grant Nos. 2014K1A1A2064569 and 2014K2A1A2044402) funded by MSIP and the Ministry of Health and Welfare (Grant No. HI13C1378). K.H.K. thanks the Korea NRF (Grant Nos. 2014R1A2A1A12067510 and 2017M3C7A1044964) funded by the MEST and Grant (1711031808/50581-2016) from the Korea Institute of Radiological and Medical Sciences funded by MSIP. Y.H. thanks the Korea NRF (Grant No. 2011-0030072) J.J. acknowledges support from the Basic Science Research Program through the Korea NRF funded by the Ministry of Education (Grant No. 2017R1D1A1B03035525).

Funders	Funder number
National Science Foundation Arctic Social Science Program	CBET-1603177, 1603177
Defense Advanced Research Projects Agency	HR0011-13-2-0017
University of California San Diego Health
Ministry of Education China	2014K1A1A2064569, 2016R1A6A3A03006343, 2014K2A1A2044402
Ministry of Science, ICT and Future Planning
Korean Ministry of Health and Welfare	2014R1A2A1A12067510, 2017M3C7A1044964, HI13C1378
National Research Foundation of Korea
Science and Technology Development Center, Ministry of Education	1711031808/50581-2016
Korea Institute of Radiological and Medical Sciences	2011-0030072, 2017R1D1A1B03035525

Keywords

cancer diagnostics
iRGD targeting peptide
nanomedicine
photoluminescence

ASJC Scopus subject areas

General Materials Science
Mechanics of Materials
Mechanical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/adma.201703309

Cite this

@article{2b9339167ea64045a84f50cf1c72c527,

title = "Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles",

abstract = "A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650–1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.",

keywords = "cancer diagnostics, iRGD targeting peptide, nanomedicine, photoluminescence",

author = "Dokyoung Kim and Jinyoung Kang and Taejun Wang and Ryu, {Hye Gun} and Zuidema, {Jonathan M.} and Jinmyoung Joo and Muwoong Kim and Youngbuhm Huh and Junyang Jung and Ahn, {Kyo Han} and Kim, {Ki Hean} and Sailor, {Michael J.}",

note = "Publisher Copyright: {\textcopyright} 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2017",

month = oct,

day = "18",

doi = "10.1002/adma.201703309",

language = "English",

volume = "29",

number = "39",

}

TY - JOUR

T1 - Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

AU - Kim, Dokyoung

AU - Kang, Jinyoung

AU - Wang, Taejun

AU - Ryu, Hye Gun

AU - Zuidema, Jonathan M.

AU - Joo, Jinmyoung

AU - Kim, Muwoong

AU - Huh, Youngbuhm

AU - Jung, Junyang

AU - Ahn, Kyo Han

AU - Kim, Ki Hean

AU - Sailor, Michael J.

PY - 2017/10/18

Y1 - 2017/10/18

N2 - A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650–1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.

AB - A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650–1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.

KW - cancer diagnostics

KW - iRGD targeting peptide

KW - nanomedicine

KW - photoluminescence

UR - http://www.scopus.com/inward/record.url?scp=85027720082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027720082&partnerID=8YFLogxK

U2 - 10.1002/adma.201703309

DO - 10.1002/adma.201703309

M3 - Article

C2 - 28833739

AN - SCOPUS:85027720082

SN - 0935-9648

VL - 29

JO - Advanced Materials

JF - Advanced Materials

IS - 39

M1 - 1703309

ER -

Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this