TY - JOUR
T1 - U1 snRNP can influence 3′-splice site selection as well as 5′-splice site selection
AU - Goguel, Valérie
AU - Liao, Xiaoling
AU - Rymond, Brian C.
AU - Rosbash, Michael
PY - 1991/8
Y1 - 1991/8
N2 - To address the mechanisms that underlie splice site selection and splice site partner assignment, we analyzed the splicing of yeast (Saccharomyces cerevisiae) transcripts containing splice site region duplications. When the 5′-splice site region was duplicated, both sites were utilized to the same extent, indicating little or no influence of proximity on 5′-splice site choice. However, the effect of a 5′-mutant site was greatly enhanced by the presence of an adjacent wild-type site, and this effect was reversed by the restoration of base-pairing with U1 snRNA. 3′-Splice site choice was apparently influenced by proximity, as the site closest to the 5′-splice site was greatly preferred. Studies with strains carrying some U1 snRNA mutations showed an increase in the use of the distal 3′-splice site, indicating a role for U1 snRNP in 3′-splice site selection. The data are compared with those from mammalian splice site choice experiments and suggest mechanisms that influence differential splice site choice as well as exon skipping.
AB - To address the mechanisms that underlie splice site selection and splice site partner assignment, we analyzed the splicing of yeast (Saccharomyces cerevisiae) transcripts containing splice site region duplications. When the 5′-splice site region was duplicated, both sites were utilized to the same extent, indicating little or no influence of proximity on 5′-splice site choice. However, the effect of a 5′-mutant site was greatly enhanced by the presence of an adjacent wild-type site, and this effect was reversed by the restoration of base-pairing with U1 snRNA. 3′-Splice site choice was apparently influenced by proximity, as the site closest to the 5′-splice site was greatly preferred. Studies with strains carrying some U1 snRNA mutations showed an increase in the use of the distal 3′-splice site, indicating a role for U1 snRNP in 3′-splice site selection. The data are compared with those from mammalian splice site choice experiments and suggest mechanisms that influence differential splice site choice as well as exon skipping.
KW - S. cerevisiae
KW - Splice site selection
KW - U1 snRNP
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M3 - Article
C2 - 1831174
AN - SCOPUS:0025951303
SN - 0890-9369
VL - 5
SP - 1430
EP - 1438
JO - Genes and Development
JF - Genes and Development
IS - 8
ER -