Ultrasound imaging of the thoracic and abdominal aorta in mice to determine aneurysm dimensions

Hisashi Sawada, Jeff Z. Chen, Bradley C. Wright, Jessica J. Moorleghen, Hong S. Lu, Alan Daugherty

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Contemporary high-resolution ultrasound instruments have sufficient resolution to facilitate the measurement of mouse aortas. These instruments have been widely used to measure aortic dimensions in mouse models of aortic aneurysms. Aortic aneurysms are defined as permanent dilations of the aorta, which occur most frequently in the ascending and abdominal regions. Sequential measurements of aortic dimensions by ultrasound are the principal approach for assessing the development and progression of aortic aneurysms in vivo. Although many reported studies used ultrasound imaging to measure aortic diameters as a primary endpoint, there are confounding factors, such as probe position and cardiac cycle, that may impact the accuracy of data acquisition, analysis, and interpretation. The purpose of this protocol is to provide a practical guide on the use of ultrasound to measure the aortic diameter in a reliable and reproducible manner. This protocol introduces the preparation of mice and instruments, the acquisition of appropriate ultrasound images, and data analysis.

Original languageEnglish
Article numbere59013
JournalJournal of Visualized Experiments
Volume2019
Issue number145
DOIs
StatePublished - Mar 2019

Bibliographical note

Publisher Copyright:
© 2019 Journal of Visualized Experiments.

Keywords

  • Abdominal aorta
  • Aorta
  • Aortic aneurysm
  • Aortic dimensions
  • Aortic sinus
  • Ascending aorta
  • Issue 145
  • Medicine
  • Ultrasound imaging

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology

Fingerprint

Dive into the research topics of 'Ultrasound imaging of the thoracic and abdominal aorta in mice to determine aneurysm dimensions'. Together they form a unique fingerprint.

Cite this