Ultrasound Monitoring of Thymus Involution in Septic Mice

Misa Ito; Qian Wang; Dan Hao; Hisashi Sawada; Bin Huang; Ling Guo; Alan Daugherty; Xiang An Li

doi:10.1016/j.ultrasmedbio.2020.11.031

Ultrasound Monitoring of Thymus Involution in Septic Mice

Misa Ito, Qian Wang, Dan Hao, Hisashi Sawada, Bin Huang, Ling Guo, Alan Daugherty, Xiang An Li

Research output: Contribution to journal › Article › peer-review

Abstract

Thymus involution is characterized by a progressive regression of thymus size and contributes to immunosuppression in sepsis. High-frequency ultrasonography is a non-invasive monitoring system in multiple organs, including the thymus, in mice. However, thymus involution has not been studied using ultrasonography in septic mice. This study reports ultrasound approaches to monitoring septic thymus involution in mice. Sepsis was induced by cecum ligation and puncture (CLP). Mice were euthanized at three time points: baseline and days 3 and 10 after CLP. Thymus areas and volumes were measured using 2-D and 3-D ultrasound approaches. Thymus weights were measured ex vivo. Compared with values at baseline, both thymus area and volume decreased significantly at days 3 and 10. In addition, thymus areas and volumes correlated positively with thymus weights. In conclusion, ultrasonography provides reliable thymus measurements and is an optimal technique for monitoring thymus involution in septic mice.

Original language	English
Pages (from-to)	769-776
Number of pages	8
Journal	Ultrasound in Medicine and Biology
Volume	47
Issue number	3
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2020.11.031
State	Published - Mar 2021

Bibliographical note

Funding Information:
This study was supported by National Institutes of Health (NIH) Grants NIH R01 GM113832, NIH R01 GM121796 and VA 1 I01 BX004639 (to X-A Li). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH or VA. This research was supported by the Shared Resource Facilities of the University of Kentucky Markey Cancer Center (P30 CA177558) and the Saha Cardiovascular Research Center.

Publisher Copyright:
© 2020 World Federation for Ultrasound in Medicine & Biology

Keywords

Mouse
Sepsis
Thymus
Thymus involution
Ultrasound

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Biophysics
Acoustics and Ultrasonics

Access to Document

10.1016/j.ultrasmedbio.2020.11.031

Cite this

@article{29a19a8557a74141b93e1a76232a2417,

title = "Ultrasound Monitoring of Thymus Involution in Septic Mice",

abstract = "Thymus involution is characterized by a progressive regression of thymus size and contributes to immunosuppression in sepsis. High-frequency ultrasonography is a non-invasive monitoring system in multiple organs, including the thymus, in mice. However, thymus involution has not been studied using ultrasonography in septic mice. This study reports ultrasound approaches to monitoring septic thymus involution in mice. Sepsis was induced by cecum ligation and puncture (CLP). Mice were euthanized at three time points: baseline and days 3 and 10 after CLP. Thymus areas and volumes were measured using 2-D and 3-D ultrasound approaches. Thymus weights were measured ex vivo. Compared with values at baseline, both thymus area and volume decreased significantly at days 3 and 10. In addition, thymus areas and volumes correlated positively with thymus weights. In conclusion, ultrasonography provides reliable thymus measurements and is an optimal technique for monitoring thymus involution in septic mice.",

keywords = "Mouse, Sepsis, Thymus, Thymus involution, Ultrasound",

author = "Misa Ito and Qian Wang and Dan Hao and Hisashi Sawada and Bin Huang and Ling Guo and Alan Daugherty and Li, {Xiang An}",

note = "Funding Information: This study was supported by National Institutes of Health (NIH) Grants NIH R01 GM113832, NIH R01 GM121796 and VA 1 I01 BX004639 (to X-A Li). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH or VA. This research was supported by the Shared Resource Facilities of the University of Kentucky Markey Cancer Center (P30 CA177558) and the Saha Cardiovascular Research Center. Publisher Copyright: {\textcopyright} 2020 World Federation for Ultrasound in Medicine & Biology",

year = "2021",

month = mar,

doi = "10.1016/j.ultrasmedbio.2020.11.031",

language = "English",

volume = "47",

pages = "769--776",

number = "3",

}

TY - JOUR

T1 - Ultrasound Monitoring of Thymus Involution in Septic Mice

AU - Ito, Misa

AU - Wang, Qian

AU - Hao, Dan

AU - Sawada, Hisashi

AU - Huang, Bin

AU - Guo, Ling

AU - Daugherty, Alan

AU - Li, Xiang An

N1 - Funding Information: This study was supported by National Institutes of Health (NIH) Grants NIH R01 GM113832, NIH R01 GM121796 and VA 1 I01 BX004639 (to X-A Li). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH or VA. This research was supported by the Shared Resource Facilities of the University of Kentucky Markey Cancer Center (P30 CA177558) and the Saha Cardiovascular Research Center. Publisher Copyright: © 2020 World Federation for Ultrasound in Medicine & Biology

PY - 2021/3

Y1 - 2021/3

N2 - Thymus involution is characterized by a progressive regression of thymus size and contributes to immunosuppression in sepsis. High-frequency ultrasonography is a non-invasive monitoring system in multiple organs, including the thymus, in mice. However, thymus involution has not been studied using ultrasonography in septic mice. This study reports ultrasound approaches to monitoring septic thymus involution in mice. Sepsis was induced by cecum ligation and puncture (CLP). Mice were euthanized at three time points: baseline and days 3 and 10 after CLP. Thymus areas and volumes were measured using 2-D and 3-D ultrasound approaches. Thymus weights were measured ex vivo. Compared with values at baseline, both thymus area and volume decreased significantly at days 3 and 10. In addition, thymus areas and volumes correlated positively with thymus weights. In conclusion, ultrasonography provides reliable thymus measurements and is an optimal technique for monitoring thymus involution in septic mice.

AB - Thymus involution is characterized by a progressive regression of thymus size and contributes to immunosuppression in sepsis. High-frequency ultrasonography is a non-invasive monitoring system in multiple organs, including the thymus, in mice. However, thymus involution has not been studied using ultrasonography in septic mice. This study reports ultrasound approaches to monitoring septic thymus involution in mice. Sepsis was induced by cecum ligation and puncture (CLP). Mice were euthanized at three time points: baseline and days 3 and 10 after CLP. Thymus areas and volumes were measured using 2-D and 3-D ultrasound approaches. Thymus weights were measured ex vivo. Compared with values at baseline, both thymus area and volume decreased significantly at days 3 and 10. In addition, thymus areas and volumes correlated positively with thymus weights. In conclusion, ultrasonography provides reliable thymus measurements and is an optimal technique for monitoring thymus involution in septic mice.

KW - Mouse

KW - Sepsis

KW - Thymus

KW - Thymus involution

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=85098105573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85098105573&partnerID=8YFLogxK

U2 - 10.1016/j.ultrasmedbio.2020.11.031

DO - 10.1016/j.ultrasmedbio.2020.11.031

M3 - Article

C2 - 33358338

AN - SCOPUS:85098105573

VL - 47

SP - 769

EP - 776

IS - 3

ER -

Ultrasound Monitoring of Thymus Involution in Septic Mice

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this