TY - JOUR
T1 - Unconjugated methoxylated catecholamine metabolites in human saliva. Quantitation methodology and comparison with plasma levels
AU - Drebing, Carla J.
AU - Freedman, Robert
AU - Waldo, Merilyne
AU - Gerhardt, Greg A.
PY - 1989/9
Y1 - 1989/9
N2 - A newly developed method for the simultaneous extraction and quantitation of the unconjugated levels of the catecholamine metabolites vanilmandelic acid (VMA), 3‐methoxy‐4‐hydroxyphenylethylene glycol (MHPG) and homovanillic acid (HVA) in plasma by high performance liquid chromatography with electrochemical detection was modified and applied to studies of human saliva. The assay had a mean coefficient of variation under 3% for each of the metabolites. Levels of plasma VMA, MHPG and HVA were measured in 28 normal subjects and compared to their saliva levels, obtained before and after stimulation by mastication. Significant correlations were found between plasma and saliva MHPG and HVA, but there was no correlation between plasma and saliva VMA. Salivary MHPG and HVA can be reproducibly assayed and may be useful tools for indications of changes in central and peripheral catecholamine metabolism.
AB - A newly developed method for the simultaneous extraction and quantitation of the unconjugated levels of the catecholamine metabolites vanilmandelic acid (VMA), 3‐methoxy‐4‐hydroxyphenylethylene glycol (MHPG) and homovanillic acid (HVA) in plasma by high performance liquid chromatography with electrochemical detection was modified and applied to studies of human saliva. The assay had a mean coefficient of variation under 3% for each of the metabolites. Levels of plasma VMA, MHPG and HVA were measured in 28 normal subjects and compared to their saliva levels, obtained before and after stimulation by mastication. Significant correlations were found between plasma and saliva MHPG and HVA, but there was no correlation between plasma and saliva VMA. Salivary MHPG and HVA can be reproducibly assayed and may be useful tools for indications of changes in central and peripheral catecholamine metabolism.
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U2 - 10.1002/bmc.1130030509
DO - 10.1002/bmc.1130030509
M3 - Article
C2 - 2804429
AN - SCOPUS:0024446476
SN - 0269-3879
VL - 3
SP - 217
EP - 220
JO - Biomedical Chromatography
JF - Biomedical Chromatography
IS - 5
ER -