Unconjugated methoxylated catecholamine metabolites in human saliva. Quantitation methodology and comparison with plasma levels

Carla J. Drebing, Robert Freedman, Merilyne Waldo, Greg A. Gerhardt

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A newly developed method for the simultaneous extraction and quantitation of the unconjugated levels of the catecholamine metabolites vanilmandelic acid (VMA), 3‐methoxy‐4‐hydroxyphenylethylene glycol (MHPG) and homovanillic acid (HVA) in plasma by high performance liquid chromatography with electrochemical detection was modified and applied to studies of human saliva. The assay had a mean coefficient of variation under 3% for each of the metabolites. Levels of plasma VMA, MHPG and HVA were measured in 28 normal subjects and compared to their saliva levels, obtained before and after stimulation by mastication. Significant correlations were found between plasma and saliva MHPG and HVA, but there was no correlation between plasma and saliva VMA. Salivary MHPG and HVA can be reproducibly assayed and may be useful tools for indications of changes in central and peripheral catecholamine metabolism.

Original languageEnglish
Pages (from-to)217-220
Number of pages4
JournalBiomedical Chromatography
Volume3
Issue number5
DOIs
StatePublished - Sep 1989

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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