Background: Nitroreductase reduces a broad range of nitroaromatics. Results: Steady-state and pre-steady-state kinetics were combined with tests for aminoaromatic product formation. Conclusion: Both half-reactions occur via a simple mechanism lacking detectable gating steps consistent with the broad substrate repertoire. Significance: Nitroreductase does not generate p-aminobenzoic acid and, therefore, appears not to reduce nitro groups to amines.
|Number of pages||12|
|Journal||Journal of Biological Chemistry|
|State||Published - May 30 2014|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology