Understanding the broad substrate repertoire of nitroreductase based on its kinetic mechanism

Warintra Pitsawong, John P. Hoben, Anne Frances Miller

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Background: Nitroreductase reduces a broad range of nitroaromatics. Results: Steady-state and pre-steady-state kinetics were combined with tests for aminoaromatic product formation. Conclusion: Both half-reactions occur via a simple mechanism lacking detectable gating steps consistent with the broad substrate repertoire. Significance: Nitroreductase does not generate p-aminobenzoic acid and, therefore, appears not to reduce nitro groups to amines.

Original languageEnglish
Pages (from-to)15203-15214
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number22
DOIs
StatePublished - May 30 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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