TY - JOUR
T1 - Understanding the sources of errors in ex vivo Hsp90 molecular imaging for rapid-on-site breast cancer diagnosis
AU - Wang, Roujia
AU - Alvarez, Daniel A.
AU - Crouch, Brian T.
AU - Pilani, Aditi
AU - Lam, Christopher
AU - Zhu, Caigang
AU - Hughes, Philip
AU - Katz, David
AU - Haystead, Timothy
AU - Ramanujam, Nirmala
N1 - Publisher Copyright:
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
PY - 2021/4
Y1 - 2021/4
N2 - Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.
AB - Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.
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UR - http://www.scopus.com/inward/citedby.url?scp=85104425919&partnerID=8YFLogxK
U2 - 10.1364/BOE.418818
DO - 10.1364/BOE.418818
M3 - Article
AN - SCOPUS:85104425919
SN - 2156-7085
VL - 12
SP - 2299
EP - 2311
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 4
ER -
