Abstract
Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.
| Original language | English |
|---|---|
| Pages (from-to) | 2299-2311 |
| Number of pages | 13 |
| Journal | Biomedical Optics Express |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2021 |
Bibliographical note
Publisher Copyright:© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Funding
National Institute of Biomedical Imaging and Bioengineering (5R01EB028148-02, 5R21EB025008-02). Acknowledgements. This work was supported by generous funding from NIH (5R21EB025008-02, 5R01EB028148-02). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | |
| National Institute of Biomedical Imaging and Bioengineering | 5R21EB025008-02, 5R01EB028148-02 |
ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics
