Overexpression of heat shock protein 90 (Hsp90) on the surface of breast cancer cells makes it an attractive molecular biomarker for breast cancer diagnosis. Before a ubiquitous diagnostic method can be established, an understanding of the systematic errors in Hsp90-based imaging is essential. In this study, we investigated three factors that may influence the sensitivity of ex vivo Hsp90 molecular imaging: time-dependent tissue viability, nonspecific diffusion of an Hsp90 specific probe (HS-27), and contact-based imaging. These three factors will be important considerations when designing any diagnostic imaging strategy based on fluorescence imaging of a molecular target on tissue samples.
|Number of pages
|Biomedical Optics Express
|Published - Apr 2021
Bibliographical noteFunding Information:
National Institute of Biomedical Imaging and Bioengineering (5R01EB028148-02, 5R21EB025008-02).
Acknowledgements. This work was supported by generous funding from NIH (5R21EB025008-02, 5R01EB028148-02). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics