Unidirectional movement of cellulose synthase complexes in arabidopsis seed coat epidermal cells deposit cellulose involved in mucilage extrusion, adherence, and ray formation

Jonathan S. Griffiths, Krešimir Šola, Rekha Kushwaha, Patricia Lam, Mizuki Tateno, Robin Young, Cătălin Voiniciuc, Gillian Dean, Shawn D. Mansfield, Seth Debolt, George W. Haughn

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

CELLULOSE SYNTHASE5 (CESA5) synthesizes cellulose necessary for seed mucilage adherence to seed coat epidermal cells of Arabidopsis (Arabidopsis thaliana). The involvement of additional CESA proteins in this process and details concerning the manner in which cellulose is deposited in the mucilage pocket are unknown. Here, we show that both CESA3 and CESA10 are highly expressed in this cell type at the time of mucilage synthesis and localize to the plasma membrane adjacent to the mucilage pocket. The isoxaben resistant1-1 and isoxaben resistant1-2 mutants affecting CESA3 show defects consistent with altered mucilage cellulose biosynthesis. CESA3 can interact with CESA5 in vitro, and green fluorescent protein-tagged CESA5, CESA3, and CESA10 proteins move in a linear, unidirectional fashion around the cytoplasmic column of the cell, parallel with the surface of the seed, in a pattern similar to that of cortical microtubules. Consistent with this movement, cytological evidence suggests that the mucilage is coiled around the columella and unwinds during mucilage extrusion to form a linear ray. Mutations in CESA5 and CESA3 affect the speed of mucilage extrusion and mucilage adherence. These findings imply that cellulose fibrils are synthesized in an ordered helical array around the columella, providing a distinct structure to the mucilage that is important for both mucilage extrusion and adherence.

Original languageEnglish
Pages (from-to)502-520
Number of pages19
JournalPlant Physiology
Volume168
Issue number2
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 American Society of Plant Biologists. All Rights Reserved.

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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