Unusual substrate and halide versatility of phenolic halogenase PltM

Shogo Mori; Allan H. Pang; Nishad Thamban Chandrika; Sylvie Garneau-Tsodikova; Oleg V. Tsodikov

doi:10.1038/s41467-019-09215-9

Unusual substrate and halide versatility of phenolic halogenase PltM

Shogo Mori, Allan H. Pang, Nishad Thamban Chandrika, Sylvie Garneau-Tsodikova, Oleg V. Tsodikov

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Controlled halogenation of chemically versatile substrates is difficult to achieve. Here we describe a unique flavin-dependent halogenase, PltM, which is capable of utilizing a wide range of halides for installation on a diverse array of phenolic compounds, including FDA-approved drugs and natural products, such as terbutaline, fenoterol, resveratrol, and catechin. Crystal structures of PltM in complex with phloroglucinol and FAD in different states yield insight into substrate recognition and the FAD recycling mechanism of this halogenase.

Original language	English
Article number	1255
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-09215-9
State	Published - Dec 1 2019

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Publisher Copyright:
© 2019, The Author(s).

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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AU - Tsodikov, Oleg V.

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AB - Controlled halogenation of chemically versatile substrates is difficult to achieve. Here we describe a unique flavin-dependent halogenase, PltM, which is capable of utilizing a wide range of halides for installation on a diverse array of phenolic compounds, including FDA-approved drugs and natural products, such as terbutaline, fenoterol, resveratrol, and catechin. Crystal structures of PltM in complex with phloroglucinol and FAD in different states yield insight into substrate recognition and the FAD recycling mechanism of this halogenase.

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Unusual substrate and halide versatility of phenolic halogenase PltM

Abstract

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ASJC Scopus subject areas

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