Unusual substrate and halide versatility of phenolic halogenase PltM

Shogo Mori; Allan H. Pang; Nishad Thamban Chandrika; Sylvie Garneau-Tsodikova; Oleg V. Tsodikov

doi:10.1038/s41467-019-09215-9

Unusual substrate and halide versatility of phenolic halogenase PltM

Shogo Mori
, Allan H. Pang
, Nishad Thamban Chandrika
, Sylvie Garneau-Tsodikova
, Oleg V. Tsodikov

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Controlled halogenation of chemically versatile substrates is difficult to achieve. Here we describe a unique flavin-dependent halogenase, PltM, which is capable of utilizing a wide range of halides for installation on a diverse array of phenolic compounds, including FDA-approved drugs and natural products, such as terbutaline, fenoterol, resveratrol, and catechin. Crystal structures of PltM in complex with phloroglucinol and FAD in different states yield insight into substrate recognition and the FAD recycling mechanism of this halogenase.

Original language	English
Article number	1255
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-09215-9
State	Published - Dec 1 2019

Bibliographical note

Publisher Copyright:
© 2019, The Author(s).

Funding

This work was supported by a NSF CAREER Award MCB-1149427 (to S.G.-T.), a grant as part of the National Center for Advancing Translational Sciences (UL1TR000117) (to S.G.-T. and O.V.T.), and by startup funds from the College of Pharmacy at the University of Kentucky (to S.G.-T. and O.V.T.). S.M. is a recipient of a 2018 long-term visit fellowship from the Yamada Science Foundation, Japan. The authors thank Dr. James J. La Clair and Prof. Michael D. Burkart from UCSD for valuable discussion and suggestions on optimization of PltM reaction conditions for halogenation. The authors are grateful to the staff of sector SER-CAT of Advanced Photon Source of the Argonne National Laboratory for support during the remote data collection.

Funders	Funder number
Yamada Science Foundation
University of Kentucky
National Science Foundation Arctic Social Science Program	MCB-1149427, 1149427
National Center for Advancing Translational Sciences (NCATS)	UL1TR000117

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General
General Physics and Astronomy

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Unusual substrate and halide versatility of phenolic halogenase PltM

Abstract

Bibliographical note

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ASJC Scopus subject areas

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