TY - JOUR
T1 - Upregulation of tumor necrosis factor-α production by retrovirally transduced human tumor-infiltrating lymphocytes using trans-retinoic acid
AU - Treisman, Jonathan
AU - Hwu, Patrick
AU - Yannelli, John R.
AU - Shafer, Gwen E.
AU - Cowherd, Robert
AU - Samid, Dvorit
AU - Rosenberg, Steven A.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1994/7
Y1 - 1994/7
N2 - The ability or retinoic acid (RA) to upregulate gene expression in human tumor-infiltrating lymphocytes (TIL) transduced with a Moloney murine leukemia virus containing the cDNA encoding tumor necrosis factor (TNF) has been studied. TNF production was increased approximately twofold after treatment with RA. This increase was dose dependent and corresponded to a rise in the level of LTR-driven mRNA measured by Northern analysis. RA did not appreciably increase transcription by the SV40 promoter or increase endogenous TNF production. The effect lasted for 3-6 days following withdrawal of RA. These studies indicate that RA can upregulate LTR-driven gene expression in TIL cells bearing retroviral vectors and may thus be of use in studies of the gene therapy of cancer.
AB - The ability or retinoic acid (RA) to upregulate gene expression in human tumor-infiltrating lymphocytes (TIL) transduced with a Moloney murine leukemia virus containing the cDNA encoding tumor necrosis factor (TNF) has been studied. TNF production was increased approximately twofold after treatment with RA. This increase was dose dependent and corresponded to a rise in the level of LTR-driven mRNA measured by Northern analysis. RA did not appreciably increase transcription by the SV40 promoter or increase endogenous TNF production. The effect lasted for 3-6 days following withdrawal of RA. These studies indicate that RA can upregulate LTR-driven gene expression in TIL cells bearing retroviral vectors and may thus be of use in studies of the gene therapy of cancer.
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U2 - 10.1006/cimm.1994.1189
DO - 10.1006/cimm.1994.1189
M3 - Article
C2 - 8025957
AN - SCOPUS:0028038044
SN - 0008-8749
VL - 156
SP - 448
EP - 457
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -
