Urine miRNAs as potential biomarkers for systemic reactions induced by exposure to embedded metal

Ivan J. Vechetti; Yuan Wen; Jessica F. Hoffman; Alexander P. Alimov; Vernieda B. Vergara; John F. Kalinich; Joanna M. Gaitens; Stella E. Hines; Melissa A. McDiarmid; John J. McCarthy; Charlotte Peterson

doi:10.2217/bmm-2021-0120

Urine miRNAs as potential biomarkers for systemic reactions induced by exposure to embedded metal

Ivan J. Vechetti
, Yuan Wen
, Jessica F. Hoffman
, Alexander P. Alimov
, Vernieda B. Vergara
, John F. Kalinich
, Joanna M. Gaitens
, Stella E. Hines
, Melissa A. McDiarmid
, John J. McCarthy
, Charlotte Peterson

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Aim: Explore the potential of urine microRNAs as biomarkers that may reflect the biological responses to pure metals embedded in skeletal muscle over time. Materials & methods: We tested a panel of military-relevant metals embedded in the gastrocnemius muscles of 3-month-old, male, Sprague-Dawley rats (n = 8/group) for a duration of 1, 3, 6 and 12 months, and performed small RNA-sequencing on the urine samples. Results: Results provide potential tissue targets affected by metal exposure and a list of unique or common urine microRNA biomarkers indicative of exposure to various metals, highlighting a complex systemic response. Conclusion: We have identified a panel of miRNAs as potential urine biomarkers to reflect the complex systemic response to embedded metal exposure.

Original language	English
Pages (from-to)	1397-1410
Number of pages	14
Journal	Biomarkers in Medicine
Volume	15
Issue number	15
DOIs	https://doi.org/10.2217/bmm-2021-0120
State	Published - Oct 2021

Bibliographical note

Publisher Copyright:
© 2021 Future Medicine Ltd.

Funding

The project described was supported by the grant Health Effects of Blast Injuries and Embedded Metal Fragments (W81XWH-16-2-0058) from the Congressionally Directed Medical Research Program (CDMRP) Peer-reviewed Medical Research Program. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

Funders	Funder number
Health Effects of Blast Injuries and Embedded Metal Fragments	W81XWH-16-2-0058
Congressionally Directed Medical Research Programs

Keywords

RNA sequencing
microRNA
military metals
skeletal muscle
urine biomarkers

ASJC Scopus subject areas

Drug Discovery
Clinical Biochemistry
Biochemistry, medical

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10.2217/bmm-2021-0120

Cite this

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title = "Urine miRNAs as potential biomarkers for systemic reactions induced by exposure to embedded metal",

abstract = "Aim: Explore the potential of urine microRNAs as biomarkers that may reflect the biological responses to pure metals embedded in skeletal muscle over time. Materials \& methods: We tested a panel of military-relevant metals embedded in the gastrocnemius muscles of 3-month-old, male, Sprague-Dawley rats (n = 8/group) for a duration of 1, 3, 6 and 12 months, and performed small RNA-sequencing on the urine samples. Results: Results provide potential tissue targets affected by metal exposure and a list of unique or common urine microRNA biomarkers indicative of exposure to various metals, highlighting a complex systemic response. Conclusion: We have identified a panel of miRNAs as potential urine biomarkers to reflect the complex systemic response to embedded metal exposure.",

keywords = "RNA sequencing, microRNA, military metals, skeletal muscle, urine biomarkers",

author = "Vechetti, \{Ivan J.\} and Yuan Wen and Hoffman, \{Jessica F.\} and Alimov, \{Alexander P.\} and Vergara, \{Vernieda B.\} and Kalinich, \{John F.\} and Gaitens, \{Joanna M.\} and Hines, \{Stella E.\} and McDiarmid, \{Melissa A.\} and McCarthy, \{John J.\} and Charlotte Peterson",

note = "Publisher Copyright: {\textcopyright} 2021 Future Medicine Ltd.",

year = "2021",

month = oct,

doi = "10.2217/bmm-2021-0120",

language = "English",

volume = "15",

pages = "1397--1410",

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TY - JOUR

T1 - Urine miRNAs as potential biomarkers for systemic reactions induced by exposure to embedded metal

AU - Vechetti, Ivan J.

AU - Wen, Yuan

AU - Hoffman, Jessica F.

AU - Alimov, Alexander P.

AU - Vergara, Vernieda B.

AU - Kalinich, John F.

AU - Gaitens, Joanna M.

AU - Hines, Stella E.

AU - McDiarmid, Melissa A.

AU - McCarthy, John J.

AU - Peterson, Charlotte

PY - 2021/10

Y1 - 2021/10

N2 - Aim: Explore the potential of urine microRNAs as biomarkers that may reflect the biological responses to pure metals embedded in skeletal muscle over time. Materials & methods: We tested a panel of military-relevant metals embedded in the gastrocnemius muscles of 3-month-old, male, Sprague-Dawley rats (n = 8/group) for a duration of 1, 3, 6 and 12 months, and performed small RNA-sequencing on the urine samples. Results: Results provide potential tissue targets affected by metal exposure and a list of unique or common urine microRNA biomarkers indicative of exposure to various metals, highlighting a complex systemic response. Conclusion: We have identified a panel of miRNAs as potential urine biomarkers to reflect the complex systemic response to embedded metal exposure.

AB - Aim: Explore the potential of urine microRNAs as biomarkers that may reflect the biological responses to pure metals embedded in skeletal muscle over time. Materials & methods: We tested a panel of military-relevant metals embedded in the gastrocnemius muscles of 3-month-old, male, Sprague-Dawley rats (n = 8/group) for a duration of 1, 3, 6 and 12 months, and performed small RNA-sequencing on the urine samples. Results: Results provide potential tissue targets affected by metal exposure and a list of unique or common urine microRNA biomarkers indicative of exposure to various metals, highlighting a complex systemic response. Conclusion: We have identified a panel of miRNAs as potential urine biomarkers to reflect the complex systemic response to embedded metal exposure.

KW - RNA sequencing

KW - microRNA

KW - military metals

KW - skeletal muscle

KW - urine biomarkers

UR - https://www.scopus.com/pages/publications/85116346024

UR - https://www.scopus.com/pages/publications/85116346024#tab=citedBy

U2 - 10.2217/bmm-2021-0120

DO - 10.2217/bmm-2021-0120

M3 - Article

C2 - 34541869

AN - SCOPUS:85116346024

SN - 1752-0363

VL - 15

SP - 1397

EP - 1410

JO - Biomarkers in Medicine

JF - Biomarkers in Medicine

IS - 15

ER -

Urine miRNAs as potential biomarkers for systemic reactions induced by exposure to embedded metal

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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