Use of a fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta

Yu Wang; Lisa A. Cassis; Sean E. Thatcher

doi:10.1007/978-1-4939-7030-8_5

Use of a fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta

Yu Wang, Lisa A. Cassis, Sean E. Thatcher

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

The use of fluorogenic substrates to measure enzymatic activity is widely used to understand function within different experimental models. ACE2 is important in understanding the balance between AngII and Ang-(1-7) and how this balance could then in turn influence hypertension or other disease outcomes. Here, we describe a method to measure ACE2 activity in abdominal aorta of hyperlipidemic mice under both saline and AngII infusion.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	61-67
Number of pages	7
DOIs	https://doi.org/10.1007/978-1-4939-7030-8_5
State	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1614
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

Activity
Angiotensin converting enzyme 2
Aorta
Enzyme
Fluorescence

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-7030-8_5

Cite this

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KW - Aorta

KW - Enzyme

KW - Fluorescence

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Use of a fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta

Abstract

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Bibliographical note

Keywords

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