Use of a fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta

Yu Wang, Lisa A. Cassis, Sean E. Thatcher

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The use of fluorogenic substrates to measure enzymatic activity is widely used to understand function within different experimental models. ACE2 is important in understanding the balance between AngII and Ang-(1-7) and how this balance could then in turn influence hypertension or other disease outcomes. Here, we describe a method to measure ACE2 activity in abdominal aorta of hyperlipidemic mice under both saline and AngII infusion.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages61-67
Number of pages7
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1614
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

  • Activity
  • Angiotensin converting enzyme 2
  • Aorta
  • Enzyme
  • Fluorescence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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