Use of a qualitative horse-side test to measure serum amyloid a in mares with experimentally induced ascending placentitis

Igor F. Canisso; Barry A. Ball; Carolyn Cray; Edward L. Squires; Mats H. Troedsson

doi:10.1016/j.jevs.2014.11.007

Use of a qualitative horse-side test to measure serum amyloid a in mares with experimentally induced ascending placentitis

Igor F. Canisso, Barry A. Ball, Carolyn Cray, Edward L. Squires, Mats H. Troedsson

Veterinary Science

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The objectives of this study were to assess (1) changes in serum amyloid A (SAA) detected by a lateral flow device (LFD) in mares with experimental placentitis and (2) to assess the correlation of this assay with a quantitative immunoassay. Horse mares carrying normal pregnancies were assigned to a control group (n = 12) and to a group with experimentally induced ascending placentitis (n = 13). Blood samples were obtained immediately before inoculation and/or initial sampling for control mares and then daily for 12days or until abortion. Concentration of SAA was analyzed using a turbidometric immunoassay. Retrospectively, samples -9, -6, -3, and 0days from abortion (DFA) were analyzed. Day 0 was defined as the day of abortion for mares in the placentitis group and the last sampling day for mares in the control group. Based on the LFD results, there were significant differences between mares in the control group and mares in the group with experimentally induced placentitis (P < .0001). There was a progressive increase in SAA from -6 to 0 DFA determined by the LFD. There were no significant differences among examiners (P > .05). The results obtained using the commercial LFD were moderately correlated (ρ = 0.51; P < .001) with the concentrations of SAA determined by the turbidometric assay. Five of 91 LFDs (i.e., 5.5%) failed to provide any reading. The LFD for determination of SAA appears to be a useful tool for quick assessment of whether inflammation is present; however, quantitative evaluation may still be warranted.

Original language	English
Pages (from-to)	54-59
Number of pages	6
Journal	Journal of Equine Veterinary Science
Volume	35
Issue number	1
DOIs	https://doi.org/10.1016/j.jevs.2014.11.007
State	Published - Jan 1 2015

Bibliographical note

Funding Information:
Funds provided by the Kentucky Thoroughbred Association / Kentucky Breeders and Owners and University of Kentucky (Albert Clay Endowment and Geoffrey Hughes Fellowship). The authors thank Kirsten Scoggin, Gabriel Davolli, Jessalyn Walter, Michelle Wynn, and Sydney Hughes for their assistance with this research project. None of the authors have any conflict of interest or relation with any third part that bias the publication of this article. The commercial lateral flow devices were donated by the manufacturer.

Publisher Copyright:
© 2015 Elsevier Inc.

Keywords

Abortion
Diagnostic marker
Inflammation
Placentitis
SAA

ASJC Scopus subject areas

Equine

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10.1016/j.jevs.2014.11.007

Cite this

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title = "Use of a qualitative horse-side test to measure serum amyloid a in mares with experimentally induced ascending placentitis",

abstract = "The objectives of this study were to assess (1) changes in serum amyloid A (SAA) detected by a lateral flow device (LFD) in mares with experimental placentitis and (2) to assess the correlation of this assay with a quantitative immunoassay. Horse mares carrying normal pregnancies were assigned to a control group (n = 12) and to a group with experimentally induced ascending placentitis (n = 13). Blood samples were obtained immediately before inoculation and/or initial sampling for control mares and then daily for 12days or until abortion. Concentration of SAA was analyzed using a turbidometric immunoassay. Retrospectively, samples -9, -6, -3, and 0days from abortion (DFA) were analyzed. Day 0 was defined as the day of abortion for mares in the placentitis group and the last sampling day for mares in the control group. Based on the LFD results, there were significant differences between mares in the control group and mares in the group with experimentally induced placentitis (P < .0001). There was a progressive increase in SAA from -6 to 0 DFA determined by the LFD. There were no significant differences among examiners (P > .05). The results obtained using the commercial LFD were moderately correlated (ρ = 0.51; P < .001) with the concentrations of SAA determined by the turbidometric assay. Five of 91 LFDs (i.e., 5.5%) failed to provide any reading. The LFD for determination of SAA appears to be a useful tool for quick assessment of whether inflammation is present; however, quantitative evaluation may still be warranted.",

keywords = "Abortion, Diagnostic marker, Inflammation, Placentitis, SAA",

author = "Canisso, {Igor F.} and Ball, {Barry A.} and Carolyn Cray and Squires, {Edward L.} and Troedsson, {Mats H.}",

note = "Funding Information: Funds provided by the Kentucky Thoroughbred Association / Kentucky Breeders and Owners and University of Kentucky (Albert Clay Endowment and Geoffrey Hughes Fellowship). The authors thank Kirsten Scoggin, Gabriel Davolli, Jessalyn Walter, Michelle Wynn, and Sydney Hughes for their assistance with this research project. None of the authors have any conflict of interest or relation with any third part that bias the publication of this article. The commercial lateral flow devices were donated by the manufacturer. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

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AU - Troedsson, Mats H.

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N2 - The objectives of this study were to assess (1) changes in serum amyloid A (SAA) detected by a lateral flow device (LFD) in mares with experimental placentitis and (2) to assess the correlation of this assay with a quantitative immunoassay. Horse mares carrying normal pregnancies were assigned to a control group (n = 12) and to a group with experimentally induced ascending placentitis (n = 13). Blood samples were obtained immediately before inoculation and/or initial sampling for control mares and then daily for 12days or until abortion. Concentration of SAA was analyzed using a turbidometric immunoassay. Retrospectively, samples -9, -6, -3, and 0days from abortion (DFA) were analyzed. Day 0 was defined as the day of abortion for mares in the placentitis group and the last sampling day for mares in the control group. Based on the LFD results, there were significant differences between mares in the control group and mares in the group with experimentally induced placentitis (P < .0001). There was a progressive increase in SAA from -6 to 0 DFA determined by the LFD. There were no significant differences among examiners (P > .05). The results obtained using the commercial LFD were moderately correlated (ρ = 0.51; P < .001) with the concentrations of SAA determined by the turbidometric assay. Five of 91 LFDs (i.e., 5.5%) failed to provide any reading. The LFD for determination of SAA appears to be a useful tool for quick assessment of whether inflammation is present; however, quantitative evaluation may still be warranted.

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Use of a qualitative horse-side test to measure serum amyloid a in mares with experimentally induced ascending placentitis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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