Use of a single-layer density centrifugation method enhances sperm quality in cryopreserved-thawed equine spermatozoa

Anja Stoll, Charles C. Love, Barry A. Ball

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The objective of this study was to improve the quality of cryopreserved-thawed equine sperm using single-layer density centrifugation (SLC). Sperm quality was assessed by DNA integrity, motility, morphology, mitochondrial membrane potential, viability, and plasma membrane alteration. The percentage of DNA-damaged sperm (expressed in % COMP) was lower (P = .001) after SLC (1.6 ± 0.5% vs. 6.8 ± 0.5%). Total sperm motility (80± 2.4% vs. 41.7 ± 2.4%) and progressive sperm motility (69.5 ± 2.9% vs. 31.5 ± 2.9%) (P < .001), as well as the percentage of morphologically normal sperm (45 ± 3.9% vs. 27.7 ± 3.9%), increased after SLC compared with control sample. In addition, the proportion of sperm with high mitochondrial membrane potential increased (81.6 ± 1.8% vs. 42.1 ± 1.8%), as did the viability of sperm (71.1 ± 2.4% vs. 39.5 ± 2.4%), after SLC compared with the control sperm. The proportion of sperm with alteration in plasma membrane structure was lower after SLC compared with control sample (6.4 ± 1.1% vs. 18.9 ± 1.1%). Overall, sperm recovery was 72.7 ± 3.6% in the control sample compared with 14.8 ± 3.6% after SLC (P < .001). We conclude that based on the sperm parameters evaluated, SLC improves the quality of cryopreserved-thawed equine spermatozoa.

Original languageEnglish
Pages (from-to)547-551
Number of pages5
JournalJournal of Equine Veterinary Science
Volume33
Issue number7
DOIs
StatePublished - Jul 2013

Bibliographical note

Funding Information:
Supported by the John P. Hughes Endowment and by the Center for Equine Health.

Funding

Supported by the John P. Hughes Endowment and by the Center for Equine Health.

FundersFunder number
John P. Hughes Endowment

    Keywords

    • Cryopreservation
    • Density centrifugation
    • Equine
    • Sperm chromatin
    • Sperm quality

    ASJC Scopus subject areas

    • Equine

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