Use of the CD107 mobilization assay reveals that cytotoxic T lymphocytes with novel MHC-Ib restriction are activated during Listeria monocytogenes infection

Denise S. McElroy, Adina M. Badstibner, Sarah E.F. D'Orazio

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Detection of cytotoxic activity by pathogen-specific T cells of unknown antigenic specificity is difficult due to the limitations of using infected cells, instead of peptide-pulsed cells, as targets. We report here that the recently described CD107 mobilization assay readily allowed for the ex vivo detection of cytotoxic T lymphocytes (CTL) with a novel MHC-Ib restriction that specifically recognized Listeria monocytogenes-infected macrophages. The CD107 mobilization assay is likely to be a useful tool for detection of CD8+ T cells that recognize a wide variety of intracellular pathogens.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalJournal of Immunological Methods
Volume328
Issue number1-2
DOIs
StatePublished - Dec 1 2007

Bibliographical note

Funding Information:
This work was supported by a grant from the National Center for Research Resources (P20 RR20171) to S.E.F.D.

Keywords

  • CD107 mobilization assay
  • CD8 T cells
  • Cytotoxic T lymphocytes
  • Listeria monocytogenes
  • MHC-Ib

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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