Detection of cytotoxic activity by pathogen-specific T cells of unknown antigenic specificity is difficult due to the limitations of using infected cells, instead of peptide-pulsed cells, as targets. We report here that the recently described CD107 mobilization assay readily allowed for the ex vivo detection of cytotoxic T lymphocytes (CTL) with a novel MHC-Ib restriction that specifically recognized Listeria monocytogenes-infected macrophages. The CD107 mobilization assay is likely to be a useful tool for detection of CD8+ T cells that recognize a wide variety of intracellular pathogens.
|Number of pages||8|
|Journal||Journal of Immunological Methods|
|State||Published - Dec 1 2007|
Bibliographical noteFunding Information:
This work was supported by a grant from the National Center for Research Resources (P20 RR20171) to S.E.F.D.
- CD107 mobilization assay
- CD8 T cells
- Cytotoxic T lymphocytes
- Listeria monocytogenes
ASJC Scopus subject areas
- Immunology and Allergy