Abstract
Detection of cytotoxic activity by pathogen-specific T cells of unknown antigenic specificity is difficult due to the limitations of using infected cells, instead of peptide-pulsed cells, as targets. We report here that the recently described CD107 mobilization assay readily allowed for the ex vivo detection of cytotoxic T lymphocytes (CTL) with a novel MHC-Ib restriction that specifically recognized Listeria monocytogenes-infected macrophages. The CD107 mobilization assay is likely to be a useful tool for detection of CD8+ T cells that recognize a wide variety of intracellular pathogens.
Original language | English |
---|---|
Pages (from-to) | 45-52 |
Number of pages | 8 |
Journal | Journal of Immunological Methods |
Volume | 328 |
Issue number | 1-2 |
DOIs | |
State | Published - Dec 1 2007 |
Bibliographical note
Funding Information:This work was supported by a grant from the National Center for Research Resources (P20 RR20171) to S.E.F.D.
Keywords
- CD107 mobilization assay
- CD8 T cells
- Cytotoxic T lymphocytes
- Listeria monocytogenes
- MHC-Ib
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology