Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

R. J. Danaher, E. F. Petricoin, D. C. Stein

Research output: Contribution to journalComment/debate

7 Scopus citations

Abstract

The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

Original languageEnglish
Pages (from-to)3428-3432
Number of pages5
JournalJournal of Bacteriology
Volume176
Issue number11
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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