The determination of protein structure and protein-protein interactions using homobifunctional crosslinking reagents with normal and N-15 labeled proteins was discussed. Recombinant CMP-NeuAc synthetase and HIV-integrase were prepared from normal and N-15 enriched media. In the case of crosslinked peptides originating from protein-protein interactions, some gain was obtained from a pattern recognition point of view, since these peptide types were observed as quartets. The identification of crosslinked peptides was confirmed by electrospray ionization (ESI)-mass spectrometry (MS)/MS using a quadrupole time-of-flight (TOF) instrument or MALDI-PSD.
|Number of pages
|Published - 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002 → Jun 6 2002
|Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
|6/2/02 → 6/6/02
ASJC Scopus subject areas