Uterine secretion from mares with post-breeding endometritis alters sperm motion characteristics in vitro

A. Alghamdi; M. H.T. Troedsson; T. Laschkewitsch; J. L. Xue

doi:10.1016/S0093-691X(01)00462-9

Uterine secretion from mares with post-breeding endometritis alters sperm motion characteristics in vitro

A. Alghamdi, M. H.T. Troedsson, T. Laschkewitsch, J. L. Xue

Veterinary Science

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Uterine secretion was collected from five normal mares during estrus by the use of a tampon. In subsequent estrus cycles, mares were inseminated with 1×10⁹ spermatozoa from a stallion of known fertility, and uterine secretion was collected randomly at 6, 12, and 24 hours after insemination. All mares had negative endometrial cytology before insemination. At the time of uterine secretion sampling, semen was collected from two stallions and extended with Kenney's extender to a concentration of 50×10⁶ spermatozoa/mL. Extended semen was diluted 2:1 with uterine secretion; semen extender; and centrifuged uterine secretion (noncellular). Samples were kept at room temperature and sperm motion characteristics (corrected motility (CMOT), progressively motile spermatozoa (PMS), and mean path velocity (MPV) were evaluated using a computer-assisted semen analyzer every 40 minutes for a total of 4 hours. Sperm motion characteristics of spermatozoa were significantly better when incubated in semen extender compared to uterine secretion (P < 0.05). The CMOT and PMS were significantly better in uterine secretion collected before, compared to after AI with the lowest values observed in samples collected at 12 hours after breeding (P < 0.05). Sperm motion characteristics of spermatozoa incubated in centrifuged uterine secretion was only slightly suppressed compared to spermatozoa incubated in semen extender, suggesting that the altered motion characteristics were mostly due to the presence of polymorphonuclear neutrophils (PMNs) in the samples. It was concluded from this study that spermatozoa can survive in inflamed uterine secretion, but that sperm motion characteristics in vitro are altered.

Original language	English
Pages (from-to)	1019-1028
Number of pages	10
Journal	Theriogenology
Volume	55
Issue number	4
DOIs	https://doi.org/10.1016/S0093-691X(01)00462-9
State	Published - Mar 1 2001

Bibliographical note

Funding Information:
Breeding-induced endometritis (BIE) in the mare is a physiological inflammation that serves to clear the uterus of excess spermatozoa, seminal plasma, and contaminants (10). It is Acknowledgments This project was supported by the Minnesota Equine Research Center, College of Veterinary Medicine, University of Minnesota with funds provided by the Minnesota Racing Commission, Minnesota Agricultural Experiment Station, and contributions from private donors.

Keywords

Endometritis
Mare
Sperm motion characteristics
Stallion
Uterine secretion

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

Access to Document

10.1016/S0093-691X(01)00462-9

Cite this

@article{f9eb9018df3e4ce4ac4c94817bfe0100,

title = "Uterine secretion from mares with post-breeding endometritis alters sperm motion characteristics in vitro",

abstract = "Uterine secretion was collected from five normal mares during estrus by the use of a tampon. In subsequent estrus cycles, mares were inseminated with 1×109 spermatozoa from a stallion of known fertility, and uterine secretion was collected randomly at 6, 12, and 24 hours after insemination. All mares had negative endometrial cytology before insemination. At the time of uterine secretion sampling, semen was collected from two stallions and extended with Kenney's extender to a concentration of 50×106 spermatozoa/mL. Extended semen was diluted 2:1 with uterine secretion; semen extender; and centrifuged uterine secretion (noncellular). Samples were kept at room temperature and sperm motion characteristics (corrected motility (CMOT), progressively motile spermatozoa (PMS), and mean path velocity (MPV) were evaluated using a computer-assisted semen analyzer every 40 minutes for a total of 4 hours. Sperm motion characteristics of spermatozoa were significantly better when incubated in semen extender compared to uterine secretion (P < 0.05). The CMOT and PMS were significantly better in uterine secretion collected before, compared to after AI with the lowest values observed in samples collected at 12 hours after breeding (P < 0.05). Sperm motion characteristics of spermatozoa incubated in centrifuged uterine secretion was only slightly suppressed compared to spermatozoa incubated in semen extender, suggesting that the altered motion characteristics were mostly due to the presence of polymorphonuclear neutrophils (PMNs) in the samples. It was concluded from this study that spermatozoa can survive in inflamed uterine secretion, but that sperm motion characteristics in vitro are altered.",

keywords = "Endometritis, Mare, Sperm motion characteristics, Stallion, Uterine secretion",

author = "A. Alghamdi and Troedsson, {M. H.T.} and T. Laschkewitsch and Xue, {J. L.}",

note = "Funding Information: Breeding-induced endometritis (BIE) in the mare is a physiological inflammation that serves to clear the uterus of excess spermatozoa, seminal plasma, and contaminants (10). It is Acknowledgments This project was supported by the Minnesota Equine Research Center, College of Veterinary Medicine, University of Minnesota with funds provided by the Minnesota Racing Commission, Minnesota Agricultural Experiment Station, and contributions from private donors.",

year = "2001",

month = mar,

day = "1",

doi = "10.1016/S0093-691X(01)00462-9",

language = "English",

volume = "55",

pages = "1019--1028",

number = "4",

}

TY - JOUR

T1 - Uterine secretion from mares with post-breeding endometritis alters sperm motion characteristics in vitro

AU - Alghamdi, A.

AU - Troedsson, M. H.T.

AU - Laschkewitsch, T.

AU - Xue, J. L.

N1 - Funding Information: Breeding-induced endometritis (BIE) in the mare is a physiological inflammation that serves to clear the uterus of excess spermatozoa, seminal plasma, and contaminants (10). It is Acknowledgments This project was supported by the Minnesota Equine Research Center, College of Veterinary Medicine, University of Minnesota with funds provided by the Minnesota Racing Commission, Minnesota Agricultural Experiment Station, and contributions from private donors.

PY - 2001/3/1

Y1 - 2001/3/1

N2 - Uterine secretion was collected from five normal mares during estrus by the use of a tampon. In subsequent estrus cycles, mares were inseminated with 1×109 spermatozoa from a stallion of known fertility, and uterine secretion was collected randomly at 6, 12, and 24 hours after insemination. All mares had negative endometrial cytology before insemination. At the time of uterine secretion sampling, semen was collected from two stallions and extended with Kenney's extender to a concentration of 50×106 spermatozoa/mL. Extended semen was diluted 2:1 with uterine secretion; semen extender; and centrifuged uterine secretion (noncellular). Samples were kept at room temperature and sperm motion characteristics (corrected motility (CMOT), progressively motile spermatozoa (PMS), and mean path velocity (MPV) were evaluated using a computer-assisted semen analyzer every 40 minutes for a total of 4 hours. Sperm motion characteristics of spermatozoa were significantly better when incubated in semen extender compared to uterine secretion (P < 0.05). The CMOT and PMS were significantly better in uterine secretion collected before, compared to after AI with the lowest values observed in samples collected at 12 hours after breeding (P < 0.05). Sperm motion characteristics of spermatozoa incubated in centrifuged uterine secretion was only slightly suppressed compared to spermatozoa incubated in semen extender, suggesting that the altered motion characteristics were mostly due to the presence of polymorphonuclear neutrophils (PMNs) in the samples. It was concluded from this study that spermatozoa can survive in inflamed uterine secretion, but that sperm motion characteristics in vitro are altered.

AB - Uterine secretion was collected from five normal mares during estrus by the use of a tampon. In subsequent estrus cycles, mares were inseminated with 1×109 spermatozoa from a stallion of known fertility, and uterine secretion was collected randomly at 6, 12, and 24 hours after insemination. All mares had negative endometrial cytology before insemination. At the time of uterine secretion sampling, semen was collected from two stallions and extended with Kenney's extender to a concentration of 50×106 spermatozoa/mL. Extended semen was diluted 2:1 with uterine secretion; semen extender; and centrifuged uterine secretion (noncellular). Samples were kept at room temperature and sperm motion characteristics (corrected motility (CMOT), progressively motile spermatozoa (PMS), and mean path velocity (MPV) were evaluated using a computer-assisted semen analyzer every 40 minutes for a total of 4 hours. Sperm motion characteristics of spermatozoa were significantly better when incubated in semen extender compared to uterine secretion (P < 0.05). The CMOT and PMS were significantly better in uterine secretion collected before, compared to after AI with the lowest values observed in samples collected at 12 hours after breeding (P < 0.05). Sperm motion characteristics of spermatozoa incubated in centrifuged uterine secretion was only slightly suppressed compared to spermatozoa incubated in semen extender, suggesting that the altered motion characteristics were mostly due to the presence of polymorphonuclear neutrophils (PMNs) in the samples. It was concluded from this study that spermatozoa can survive in inflamed uterine secretion, but that sperm motion characteristics in vitro are altered.

KW - Endometritis

KW - Mare

KW - Sperm motion characteristics

KW - Stallion

KW - Uterine secretion

UR - http://www.scopus.com/inward/record.url?scp=0035280548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035280548&partnerID=8YFLogxK

U2 - 10.1016/S0093-691X(01)00462-9

DO - 10.1016/S0093-691X(01)00462-9

M3 - Article

C2 - 11291908

AN - SCOPUS:0035280548

SN - 0093-691X

VL - 55

SP - 1019

EP - 1028

JO - Theriogenology

JF - Theriogenology

IS - 4

ER -

Uterine secretion from mares with post-breeding endometritis alters sperm motion characteristics in vitro

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this