TY - JOUR
T1 - Validation of an HPLC method for analysis of DB-67 and its water soluble prodrug in mouse plasma
AU - Horn, Jamie
AU - Jordan, Sherri L.
AU - Song, Lin
AU - Roberts, Michael J.
AU - Anderson, Bradley D.
AU - Leggas, Markos
PY - 2006/11/21
Y1 - 2006/11/21
N2 - A method for the quantitation of DB-67 ((20S)-10-hydroxy-7-tert-butyldimethylsilylcamptothecin) lactone and carboxylate in mouse plasma has been developed, validated, and applied in pharmacokinetic studies. The analytes were separated by reversed-phase chromatography with fluorescence detection. Validation demonstrated the selectivity and specificity for the carboxylate and lactone, with linearity between 1-300 ng/mL and 2.5-300 ng/mL for the carboxylate and lactone, respectively (accuracy 90-110% of theory and coefficient of variation ≤5.7%). Carboxylate to lactone conversion was <4% using this method. The assay was found to be suitable for the analysis of DB-67 lactone and carboxylate in pharmacokinetic studies following intravenous administration of DB-67 or its δ-aminobutyric acid ester derivative.
AB - A method for the quantitation of DB-67 ((20S)-10-hydroxy-7-tert-butyldimethylsilylcamptothecin) lactone and carboxylate in mouse plasma has been developed, validated, and applied in pharmacokinetic studies. The analytes were separated by reversed-phase chromatography with fluorescence detection. Validation demonstrated the selectivity and specificity for the carboxylate and lactone, with linearity between 1-300 ng/mL and 2.5-300 ng/mL for the carboxylate and lactone, respectively (accuracy 90-110% of theory and coefficient of variation ≤5.7%). Carboxylate to lactone conversion was <4% using this method. The assay was found to be suitable for the analysis of DB-67 lactone and carboxylate in pharmacokinetic studies following intravenous administration of DB-67 or its δ-aminobutyric acid ester derivative.
KW - Camptothecin analogs
KW - DB-67
KW - Method validation
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U2 - 10.1016/j.jchromb.2006.06.022
DO - 10.1016/j.jchromb.2006.06.022
M3 - Article
C2 - 16860004
AN - SCOPUS:33845972223
SN - 1570-0232
VL - 844
SP - 15
EP - 22
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -