Validation of an HPLC method for analysis of DB-67 and its water soluble prodrug in mouse plasma

Jamie Horn, Sherri L. Jordan, Lin Song, Michael J. Roberts, Bradley D. Anderson, Markos Leggas

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A method for the quantitation of DB-67 ((20S)-10-hydroxy-7-tert-butyldimethylsilylcamptothecin) lactone and carboxylate in mouse plasma has been developed, validated, and applied in pharmacokinetic studies. The analytes were separated by reversed-phase chromatography with fluorescence detection. Validation demonstrated the selectivity and specificity for the carboxylate and lactone, with linearity between 1-300 ng/mL and 2.5-300 ng/mL for the carboxylate and lactone, respectively (accuracy 90-110% of theory and coefficient of variation ≤5.7%). Carboxylate to lactone conversion was <4% using this method. The assay was found to be suitable for the analysis of DB-67 lactone and carboxylate in pharmacokinetic studies following intravenous administration of DB-67 or its δ-aminobutyric acid ester derivative.

Original languageEnglish
Pages (from-to)15-22
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume844
Issue number1
DOIs
StatePublished - Nov 21 2006

Keywords

  • Camptothecin analogs
  • DB-67
  • Method validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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