Validation of dried blood spots for capturing hepatitis C virus diversity for genomic surveillance

Damien C. Tully, Karen A. Power, Jacklyn Sarette, Thomas J. Stopka, Peter D. Friedmann, P. Todd Korthuis, Hannah Cooper, April M. Young, David W. Seal, Ryan P. Westergaard, Todd M. Allen

Research output: Contribution to journalArticlepeer-review

Abstract

Dried blood spots (DBS) have emerged as a promising alternative to traditional venous blood for hepatitis C virus (HCV) testing. However, their capacity to accurately reflect the genetic diversity of HCV remains poorly understood. We employed deep sequencing and advanced phylogenetic analyses on paired plasma and DBS samples from two common subtypes to evaluate the suitability of DBS for genomic surveillance. Results demonstrated that DBS captured equivalent viral diversity compared to plasma with no phylogenetic discordance observed. The ability of DBS to accurately reflect the profile of viral genetic diversity suggests it may be a promising avenue for future surveillance efforts to curb HCV outbreaks.

Original languageEnglish
Pages (from-to)266-270
Number of pages5
JournalJournal of Viral Hepatitis
Volume31
Issue number5
DOIs
StatePublished - May 2024

Bibliographical note

Publisher Copyright:
© 2024 John Wiley & Sons Ltd.

Keywords

  • DBS
  • HCV
  • genetic diversity
  • molecular epidemiology
  • phylogenetic

ASJC Scopus subject areas

  • Hepatology
  • Infectious Diseases
  • Virology

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