VAMP3 and VAMP8 Regulate the Development and Functionality of Parasitophorous Vacuoles Housing Leishmania amazonensis

Olivier Séguin, Linh Thuy Mai, Hamlet Acevedo Ospina, Marie Michèle Guay-Vincent, Sidney W. Whiteheart, Simona Stäger, Albert Descoteaux

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

To colonize mammalian phagocytic cells, the parasite Leishmania remodels phagosomes into parasitophorous vacuoles that can be either tight-fitting individual or communal. The molecular and cellular bases underlying the biogenesis and functionality of these two types of vacuoles are poorly understood. In this study, we investigated the contribution of host cell soluble N-ethylmaleimide-sensitive-factor attachment protein receptor proteins to the expansion and functionality of communal vacuoles as well as the replication of the parasite. The differential patterns of recruitment of soluble N-ethylmaleimide-sensitive-factor attachment protein receptor to communal vacuoles harboring Leishmania amazonensis and to individual vacuoles housing L. major led us to further investigate the roles of VAMP3 and VAMP8 in the interaction of Leishmania with its host cell. We show that whereas VAMP8 contributes to the optimal expansion of communal vacuoles, VAMP3 negatively regulates L. amazonensis replication, vacuole size, as well as antigen cross-presentation. In contrast, neither protein has an impact on the fate of L. major. Collectively, our data support a role for both VAMP3 and VAMP8 in the development and functionality of L. amazonensis-harboring communal parasitophorous vacuoles.

Original languageEnglish
Article numbere00183-21
JournalInfection and Immunity
Volume90
Issue number3
DOIs
StatePublished - Mar 2022

Bibliographical note

Funding Information:
This work was supported by Canadian Institutes of Health Research (CIHR) grants PJT-156416 to A.D. and PJT-159647 to S.S. A.D. is the holder of the Canada Research Chair on the Biology of Intracellular Parasitism. O.S. was supported by a doctoral award from the Fonds de Recherche du Québec-Santé. L.T.M. was supported by a studentship from the Fondation Armand-Frappier. The funders had no role in study design, data collection and interpretation, decision to publish, or preparation of the manuscript. We declare no conflicts of interest.

Publisher Copyright:
Copyright © 2022 American Society for Microbiology. All Rights Reserved.

Keywords

  • Cross-presentation
  • Leishmania
  • Macrophage
  • Parasitophorous vacuole
  • SNARE

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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