Vanadate activated Akt and promoted S phase entry

Zhuo Zhang, Ning Gao, Hengjun He, Chuanshu Huang, Jia Luo, Xianglin Shi

Research output: Contribution to journalReview articlepeer-review

30 Scopus citations


Protein kinase B (PKB)/Akt and its upstream signal transducer, phosphatidylinosito-3 kinase (PI3K) play an essential role in control of transcription and translation, which impact cell growth, survival, and metabolism. Transcription factor E2F is a component of the downstream proliferative machinery regulated by Akt. Hyperphosphorylation of retinoblastoma protein (pRb), a pocket protein, leads to release of E2F1, resulting in transition from G1 to S phase. The present study shows that in normal C141 cells, vanadate treatment increased the percentage of cells at S phase and elevated cyclin E and cyclin A expression. Vanadate treatment triggered phosphorylation of pRb and release of E2F1. Furthermore, vanadate increased Akt kinase activity and caused its phosphorylation at Ser473 and Thr308. Inhibition of Akt by either inhibitors or transfected cells with dominant negative kinase mutant or dominant negative phosphorylation mutant decreased the percentage of the cells at the S phase induced by vanadate, and reduced both cyclin E and E2F1 expression and phosphorylation of pRb. The present study indicates that Akt plays an essential role in vanadate-induced increase in cell number at S phase and transition from G1 to S phase through E2F-pRb pathway.

Original languageEnglish
Pages (from-to)227-237
Number of pages11
JournalMolecular and Cellular Biochemistry
Issue number1-2
StatePublished - Jan 2004


  • Akt
  • Cell cycle regulation
  • E2F1
  • PI3k
  • Vanadate
  • pRb

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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