Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds

Runzhi Li; Keshun Yu; Yongmei Wu; Mizuki Tateno; Tomoko Hatanaka; David F. Hildebrand

doi:10.1016/j.ymben.2011.11.004

Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds

Runzhi Li, Keshun Yu, Yongmei Wu, Mizuki Tateno, Tomoko Hatanaka, David F. Hildebrand

Plant and Soil Sciences

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Plant oils can be useful chemical feedstocks such as a source of epoxy fatty acids. High seed-specific expression of a Stokesia laevis epoxygenase (SlEPX) in soybeans only results in 3-7% epoxide levels. SlEPX-transgenic soybean seeds also exhibited other phenotypic alterations, such as altered seed fatty acid profiles, reduced oil accumulation, and variable protein levels. SlEPX-transgenic seeds showed a 2-5% reduction in total oil content and protein levels of 30.9-51.4%. To address these pleiotrophic effects of SlEPX expression on other traits, transgenic soybeans were developed to co-express SlEPX and DGAT (diacylglycerol acyltransferase) genes (VgDGAT1 & 2) isolated from Vernonia galamensis, a high accumulator of epoxy fatty acids. These side effects of SlEPX expression were largely overcome in the DGAT co-expressing soybeans. Total oil and protein contents were restored to the levels in non-transgenic soybeans, indicating that both VgDGAT1 and VgDGAT2 could complement the disrupted phenotypes caused by over-expression of an epoxygenase in soybean seeds.

Original language	English
Pages (from-to)	29-38
Number of pages	10
Journal	Metabolic Engineering
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/j.ymben.2011.11.004
State	Published - Jan 2012

Bibliographical note

Funding Information:
This work was supported by the Consortium for Plant Biotechnology Research (CPBR) , the United Soybean Board (USB) , Ashland Chemicals and the Kentucky Agricultural Experiment Station . We thank Scott Serdoz, Dennis Egli and Todd Pfeiffer for help with the field production of the soybeans, John Johnson for fatty acid analysis and Bob Geneve for help with pictures.

Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.

Keywords

Acyltransferase
Epoxide
Glycine max
Seed development
Triacylglycerol

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.ymben.2011.11.004

Cite this

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title = "Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds",

abstract = "Plant oils can be useful chemical feedstocks such as a source of epoxy fatty acids. High seed-specific expression of a Stokesia laevis epoxygenase (SlEPX) in soybeans only results in 3-7% epoxide levels. SlEPX-transgenic soybean seeds also exhibited other phenotypic alterations, such as altered seed fatty acid profiles, reduced oil accumulation, and variable protein levels. SlEPX-transgenic seeds showed a 2-5% reduction in total oil content and protein levels of 30.9-51.4%. To address these pleiotrophic effects of SlEPX expression on other traits, transgenic soybeans were developed to co-express SlEPX and DGAT (diacylglycerol acyltransferase) genes (VgDGAT1 & 2) isolated from Vernonia galamensis, a high accumulator of epoxy fatty acids. These side effects of SlEPX expression were largely overcome in the DGAT co-expressing soybeans. Total oil and protein contents were restored to the levels in non-transgenic soybeans, indicating that both VgDGAT1 and VgDGAT2 could complement the disrupted phenotypes caused by over-expression of an epoxygenase in soybean seeds.",

keywords = "Acyltransferase, Epoxide, Glycine max, Seed development, Triacylglycerol",

author = "Runzhi Li and Keshun Yu and Yongmei Wu and Mizuki Tateno and Tomoko Hatanaka and Hildebrand, {David F.}",

note = "Funding Information: This work was supported by the Consortium for Plant Biotechnology Research (CPBR) , the United Soybean Board (USB) , Ashland Chemicals and the Kentucky Agricultural Experiment Station . We thank Scott Serdoz, Dennis Egli and Todd Pfeiffer for help with the field production of the soybeans, John Johnson for fatty acid analysis and Bob Geneve for help with pictures. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2012",

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doi = "10.1016/j.ymben.2011.11.004",

language = "English",

volume = "14",

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AU - Yu, Keshun

AU - Wu, Yongmei

AU - Tateno, Mizuki

AU - Hatanaka, Tomoko

AU - Hildebrand, David F.

N1 - Funding Information: This work was supported by the Consortium for Plant Biotechnology Research (CPBR) , the United Soybean Board (USB) , Ashland Chemicals and the Kentucky Agricultural Experiment Station . We thank Scott Serdoz, Dennis Egli and Todd Pfeiffer for help with the field production of the soybeans, John Johnson for fatty acid analysis and Bob Geneve for help with pictures. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2012/1

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N2 - Plant oils can be useful chemical feedstocks such as a source of epoxy fatty acids. High seed-specific expression of a Stokesia laevis epoxygenase (SlEPX) in soybeans only results in 3-7% epoxide levels. SlEPX-transgenic soybean seeds also exhibited other phenotypic alterations, such as altered seed fatty acid profiles, reduced oil accumulation, and variable protein levels. SlEPX-transgenic seeds showed a 2-5% reduction in total oil content and protein levels of 30.9-51.4%. To address these pleiotrophic effects of SlEPX expression on other traits, transgenic soybeans were developed to co-express SlEPX and DGAT (diacylglycerol acyltransferase) genes (VgDGAT1 & 2) isolated from Vernonia galamensis, a high accumulator of epoxy fatty acids. These side effects of SlEPX expression were largely overcome in the DGAT co-expressing soybeans. Total oil and protein contents were restored to the levels in non-transgenic soybeans, indicating that both VgDGAT1 and VgDGAT2 could complement the disrupted phenotypes caused by over-expression of an epoxygenase in soybean seeds.

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KW - Glycine max

KW - Seed development

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Vernonia DGATs can complement the disrupted oil and protein metabolism in epoxygenase-expressing soybean seeds

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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